Strain Data Sheet

RBRC00986

Strain Information

Image
BRC No.RBRC00986
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6C3F1-Tg(CAG-EGFP)CX-FM50Osb
Former Common nameB6C3F1B6C3F1 TgN(act-EGFP)OsbCX-FM050
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Masaru Okabe at Osaka University in 1997. The CAG-GFP plasmids were injected into the pronuclei of B6C3F2 fertilized eggs.
Strain descriptionBoth homozygous and hemizygous transgenic mice express GFP in all over the body. The transgene is located in X chromosome. This strain is useful for detection of cell fate in transplantation and developmental studies.
Colony maintenanceSibling Mating (Homozygote x Hemizygote)
References
'Green mice' as a source of ubiquitous green cells.
Okabe M, Ikawa M, Kominami K, Nakanishi T, Nishimune Y
FEBS Lett., 407, 313-319 (1997). 9175875

FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Nakanishi T, Kuroiwa A, Yamada S, Isotani A, Yamashita A, Tairaka A, Hayashi T, Takagi T, Ikawa M, Matsuda Y, Okabe M
Genomics, 80, 564-574 (2002). 12504848

Genomic imprinting of XX spermatogonia and XX oocytes recovered from XX<-->XY chimeric testes.
Isotani A, Nakanishi T, Kobayashi S, Lee J, Chuma S, Nakatsuji N, Ishino F, Okabe M
Proc. Natl. Acad. Sci. USA, 102(11), 4039-4044 (2005). 15746241

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Green Fluorescent Protein (Aequorea victoria)XEGFP CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • cellular phenotype(MP:0005384)

  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genomics, 80, 564-574 (2002). Proc. Natl. Acad. Sci. USA, 102(11), 4039-4044 (2005). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasaru Okabe (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data