Strain Data Sheet

RBRC00989

Strain Information

Image
BRC No.RBRC00989
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gad2<tm1Oba>/CRbrc
Former Common nameGAD65 Knockout mice (C line)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
A novel ES cell line, TT2, with high germline-differentiating potency.
Yagi T, Tokunaga T, Furuta Y, Nada S, Yoshida M, Tsukada T, Saga Y, Takeda N, Ikawa Y, Aizawa S
Anal. Biochem., 214, 70-76 (1993). 8250257

Mice lacking the 65 kDa isoform of glutamic acid decarboxylase (GAD65) maintain normal levels of GAD67 and GABA in their brains but are susceptible to seizures.
Asada H, Kawamura Y, Maruyama K, Kume H, Ding R, Ji F Y, Kanbara N, Kuzume H, Sanbo M, Yagi T, Obata K
Biochem. Biophys. Res. Commun., 229, 891-895 (1996). 8954991

Synaptic localization of the 67,000 mol. wt isoform of glutamate decarboxylase and transmitter function of GABA in the mouse cerebellum lacking the 65,000 mol. wt isoform.
Obata K, Fukuda T, Konishi S, Ji F Y, Mitoma H, Kosaka T
Neuroscience, 93, 1475-1482 (1999). 10501472

Postnatal development of a GABA deficit and disturbance of neural functions in mice lacking GAD65.
Stork O, Ji F Y, Kaneko K, Stork S, Yoshinobu Y, Moriya T, Shibata S, Obata K
Brain Res., 865, 45-58 (2000). 10814732

Altered conditioned fear behavior in glutamate decarboxylase 65 null mutant mice.
Stork O, Yamanaka H, Stork S, Kume N, Obata K
Genes Brain Behav., 2, 65-70 (2003). 12884963

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gad2
MGI:95634		glutamic acid decarboxylase 22Gad2<tm1Oba>
MGI:2177567		targeted mutation 1, Kunihiko Obata
neo neomycin resistance gene (E. coli)2 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal synaptic neurotransmitter level(MP:0002204)

  • decreased aggression towards male mice(MP:0003862)

  • decreased gamma-aminobutyric acid level(MP:0030733)

  • impaired swimming(MP:0001522)

  • increased anxiety-related response(MP:0001363)
    • more 5 phenotypes
  • increased coping response(MP:0003063)

  • myoclonus(MP:0000243)

  • premature death(MP:0002083)

  • seizures(MP:0002064)

  • tonic-clonic seizures(MP:0003997)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse GAD65 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), poly A
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 229, 891-895 (1996).
    DepositorKunihiko Obata (National Institute for Physiological Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data