Strain Data Sheet

RBRC00990

Strain Information

Image
BRC No.RBRC00990
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gad1<tm1Oba>/Rbrc
Former Common nameGAD67 Knockout mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
A novel ES cell line, TT2, with high germline-differentiating potency.
Yagi T, Tokunaga T, Furuta Y, Nada S, Yoshida M, Tsukada T, Saga Y, Takeda N, Ikawa Y, Aizawa S
Anal. Biochem., 214, 70-76 (1993). 8250257

Cleft palate and decreased brain gamma-aminobutyric acid in mice lacking the 67-kDa isoform of glutamic acid decarboxylase.
Asada H, Kawamura Y, Maruyama K, Kume H, Ding R G, Kanbara N, Kuzume H, Sanbo M, Yagi T, Obata K
Proc. Natl. Acad. Sci. USA, 94, 6496-6499 (1997). 9177246

GABA and histogenesis in fetal and neonatal mouse brain lacking both the isoforms of glutamic acid decarboxylase.
Ji F, Kanbara N, Obata K
Neurosci. Res., 33, 187-194 (1999). 10211762

Development of the GABA system in organotypic culture of hippocampal and cerebellar slices from a 67-kDa isoform of glutamic acid decarboxylase (GAD67)-deficient mice.
Ji F, Obata K
Neurosci. Res., 33, 233-237 (1999). 10211768

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gad1
MGI:95632		glutamate decarboxylase 12Gad1<tm1Oba>
MGI:2177565		targeted mutation 1, Kunihiko Obata
  • developmental and epileptic encephalopathy 89(MedGEN)
    • neo neomycin resistance gene (E. coli)2 mouse phosphoglycerate kinase promoter (PGK promoter)
    lacZ beta-galactosidase (E. coli)UN

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal enzyme/coenzyme activity(MP:0005584)

  • abnormal synaptic neurotransmitter level(MP:0002204)

  • absent gastric milk in neonates(MP:0009546)

  • atelectasis(MP:0001177)

  • cleft palate(MP:0000111)
    • more 6 phenotypes
  • cyanosis(MP:0001575)

  • decreased gamma-aminobutyric acid level(MP:0030733)

  • decreased glutamate decarboxylase activity(MP:0012595)

  • distended abdomen(MP:0001270)

  • neonatal lethality, complete penetrance(MP:0011087)

  • respiratory distress(MP:0001954)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse GAD67 genomic DNA, E. coli neo, E. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), poly A
    Research applicationNeurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA., 94, 6496-6499 (1997).
    DepositorKunihiko Obata (National Institute for Physiological Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data