Strain Data Sheet

RBRC00994

Strain Information

Image
BRC No.RBRC00994
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Sh2b1<tm1Ayos>/Rbrc
Former Common nameSH2-B knockout mice, C57BL/6.129 SH2-B -/-
H-2 Haplotype
ES Cell lineAK7 [129S4/SvJaeSor]
Background strainC57BL/6 and 129 mixed
Appearance
Strain developmentC57BL/6 X 129 mix background
Strain description
Colony maintenance
References
Mice lacking the homologue of the human 22q11.2 gene CRKL phenocopy neurocristopathies of DiGeorge syndrome.
Guris D L, Fantes J, Tara D, Druker B J, Imamoto A
Nat. Genet., 27, 293-298 (2001). 11242111

SH2-B is required for both male and female reproduction.
Ohtsuka S, Takaki S, Iseki M, Miyoshi K, Nakagata N, Kataoka Y, Yoshida N, Takatsu K, Yoshimura A
Mol. Cell. Biol., 22, 3066-3077 (2002). 11940664

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sh2bpsm1
MGI:1201407		SH2-B PH domain containing signaling mediator 17Sh2bpsm1 <tm1Ayos>
MGI:2662436		targeted mutation 1, Akihiko Yoshimura
neo neomycin resistance gene (E. coli)7

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal epididymis morphology (MP:0002631)

  • abnormal oocyte morphology (MP:0001125)

  • abnormal ovarian folliculogenesis (MP:0001130)

  • abnormal ovulation (MP:0001928)

  • abnormal uterus development (MP:0003572)
    • more 15 phenotypes
  • asthenozoospermia (MP:0002675)

  • decreased Leydig cell number (MP:0005536)

  • decreased body weight (MP:0001262)

  • decreased ovary weight (MP:0004856)

  • decreased testis weight (MP:0004852)

  • endocrine/exocrine gland phenotype (MP:0005379)

  • immune system phenotype (MP:0005387)

  • neonatal lethality, incomplete penetrance (MP:0011088)

  • oligozoospermia (MP:0002687)

  • ovary hypoplasia (MP:0005158)

  • postnatal growth retardation (MP:0001732)

  • proportional dwarf (MP:0008974)

  • reduced female fertility (MP:0001923)

  • reduced male fertility (MP:0001922)

  • testis hypoplasia (MP:0001940)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse SH2-B PH domain containing signaling mediator genomic DNA, E. coli neo
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 22, 3066-3077 (2002). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The depositor should be a co-author in the articles when the users publish their data using these mice for 1 year after provided.
    Users should contact the depositor in the case of application for any patents with the results from these mice.
    DepositorSatoshi Takaki (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Wu G, Liu Y, Huang H, Tang Y, Liu W, Mei Y, Wan N, Liu X, Huang C.
    SH2B1 is critical for the regulation of cardiac remodelling in response to pressure overload.
    Cardiovasc Res 107(2) 203-15(2015) 26077624