Strain Information | |
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Image | ![]() |
BRC No. | RBRC00995 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6.129P2-Il5ra<tm1Ktk>/KtkRbrc |
Former Common name | C57BL/6 IL-5R alpha -/- |
H-2 Haplotype | |
ES Cell line | E14.1 [129P2/OlaHsd] |
Background strain | C57BL/6NCrSlc |
Appearance | |
Strain development | Developed by Kiyoshi Takatsu, Institute of Medical Science in 1996. E14.1 ES cells were used. The mutant mice were backcrossed to C57BL/6CrSlc. |
Strain description | L-5Ralpha gene knockout mice. A translational stop codon was introduced into exon 5 of the Il5ra and the exon 6 was replaced with a neo cassette. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc] |
References | Defective B-1 cell development and impaired immunity against Angiostrongylus cantonensis in IL-5R alpha-deficient mice. Yoshida T, Ikuta K, Sugaya H, Maki K, Takagi M, Kanazawa H, Sunaga S, Kinashi T, Yoshimura K, Miyazaki J, Takaki S, Takatsu K Immunity, 4, 483-494 (1996). 8630733Functional dissection of the cytoplasmic subregions of the interleukin-5 receptor alpha chain in growth and immunoglobulin G1 switch recombination of B cells. Moon B G, Yoshida T, Shiiba M, Nakao K, Katsuki M, Takaki S, Takatsu K Immunology, 102, 289-300 (2001). 11298827 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Il5ra MGI:96558 | interleukin 5 receptor, alpha | 6 | Il5ra<tm1Ktk> MGI:2180501 | targeted mutation 1, Kiyoshi Takatsu | |||
neo | neomycin resistance gene (E. coli) | 6 | mouse phosphoglycerate kinase promoter (PGK promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 11 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse IL-5R α genomic DNA |
Research application | Immunology and Inflammation Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity 4, 483-494 (1996). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The depositor should be a co-author in the articles when the users publish their data using these mice for 1 year after provided. Users should contact the depositor in the case of application for any patents with the results from these mice. |
Depositor | Kiyoshi Takatsu (The University of Tokyo) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Lee D, Jo MG, Min KY, Choi MY, Kim YM, Kim HS, Choi WS. IL-10+ regulatory B cells mitigate atopic dermatitis by suppressing eosinophil activation. Sci Rep 14(1) 18164(2024) 39107352 |
Kim HS, Lee MB, Lee D, Min KY, Koo J, Kim HW, Park YH, Kim SJ, Ikutani M, Takaki S, Kim YM, Choi WS. The regulatory B cell-mediated peripheral tolerance maintained by mast cell IL-5 suppresses oxazolone-induced contact hypersensitivity. Sci Adv 5(7) eaav8152(2019) 31328158 |