Strain Data Sheet

RBRC00999

Strain Information

Image
BRC No.RBRC00999
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(Plp1)1Kmik/Rbrc
Former Common namePLP-Tg
H-2 Haplotype
ES Cell line
Background strainCrlj:B6D2F1
Appearance
Strain developmentDeveloped by Kazuhiro Ikenaka, Osaka University in 1989.
Strain descriptionThese ransgenic mice overexpress the Plp gene by introducing extra wild-type mouse proteolipid protein, PLP genes. Tg homozygous mice with 4 more Plp genes show dysmyelination in the CNS and die within about 1 month after birth. Whereas Tg hemizygous with 2 more Plp genes show normal myelination at an early age (3 weeks after birth), but later developed demyelination.
Colony maintenance
References
Glial cell degeneration and hypomyelination caused by overexpression of myelin proteolipid protein gene.
Kagawa T, Ikenaka K, Inoue Y, Kuriyama S, Tsujii T, Nakao J, Nakajima K, Aruga J, Okano H, Mikoshiba K
Neuron, 13, 427-442 (1994). 7520255

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Plp1 proteolipid protein (myelin) 1UNPlp1
MGI:4354124		transgene insertion 1, Katsuhiko Mikoshibamouse proteolipid protein (myelin) promoter (20 kb)
neo neomycin resistance gene (E. coli)UN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal Golgi apparatus morphology(MP:0011743)

  • abnormal oligodendrocyte morphology(MP:0000953)

  • ataxia(MP:0001393)

  • convulsive seizures(MP:0000947)

  • demyelination(MP:0000921)
    • more 3 phenotypes
  • dysmyelination(MP:0013438)

  • postnatal lethality(MP:0002082)

  • tremors(MP:0000745)
    • Detailed phenotype data

    Ordering Information

    Donor DNAC57BL/6 mouse Plp1 genomic DNA, cosmid vector LoristB (E. coli neo)
    Research applicationNeurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 13, 427-442 (1994).
    DepositorKazuhiro Ikenaka (National Institutes of Natural Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    Miyamoto Y, Tanaka M, Ito H, Ooizumi H, Ohbuchi K, Mizoguchi K, Torii T, Yamauchi J.
    Expression of kinase-deficient MEK2 ameliorates Pelizaeus-Merzbacher disease phenotypes in mice.
    Biochem Biophys Res Commun 531(4) 445-451(2020) 32800341