Strain Data Sheet

RBRC01058

Strain Information

Image
BRC No.RBRC01058
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Nrp1<tm1Hfu>/HfuRbrc
Former Common nameICR-Nrp1<tmHfu>
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainICR
Appearancealbino
Strain developmentDeveloped by Dr. Hajime Fujisawa, Nagoya University in 1997. A neomycin selection cassette was inserted into a fragment of the sequence encoding the a1 domain of the protein. ICR background.
Strain descriptionICR.Cg-Nrp1<tm1Hfu>. Neuropilin-1 (Nrp1) is a type I membrane protein and has been shown to function as a receptor for semaphorin III/D. Nrp1 homozygous mutant mice were embryonic lethal, and show severe abnormalities in the trajectory of the cranial and spinal efferent fibers of the parasympathetic nerve system.
Colony maintenanceSibling Mating (Heterozygote x Wild-type, Heterozygote) or Cross to ICR (Heterozygote x Slc:ICR)Embryonic lethality (Homozygous). Homozygote mutant mice die up to E11.
References
Neuropilin-semaphorin III/D-mediated chemorepulsive signals play a crucial role in peripheral nerve projection in mice.
Kitsukawa T, Shimizu M, Sanbo M, Hirata T, Taniguchi M, Bekku Y, Yagi T, Fujisawa H
Neuron, 19, 995-1005 (1997). 9390514

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Nrp1
MGI:106206		neuropilin 18Nrp1<tm1Hfu>
MGI:1857853		targeted mutation 1, Hajime Fujisawa
neo neomycin resistance gene (E. coli)8 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal aortic arch and aortic arch branch attachment(MP:0010464)

  • abnormal axon guidance(MP:0002961)

  • abnormal brain vasculature morphology(MP:0004950)

  • abnormal facial motor nucleus morphology(MP:0000909)

  • abnormal facial nerve morphology(MP:0001071)
    • more 20 phenotypes
  • abnormal first pharyngeal arch artery morphology(MP:0010355)

  • abnormal fourth pharyngeal arch artery morphology(MP:0006354)

  • abnormal neuronal migration(MP:0006009)

  • abnormal pharyngeal arch artery morphology(MP:0002672)

  • abnormal pulmonary artery morphology(MP:0000484)

  • abnormal second pharyngeal arch artery morphology(MP:0010356)

  • abnormal sixth pharyngeal arch artery morphology(MP:0006355)

  • abnormal spinal nerve morphology(MP:0001077)

  • abnormal third pharyngeal arch artery morphology(MP:0006356)

  • abnormal trigeminal nerve morphology(MP:0001065)

  • abnormal vascular development(MP:0000259)

  • decreased angiogenesis(MP:0005602)

  • decreased neuron number(MP:0008948)

  • disorganized yolk sac vascular plexus(MP:0001723)

  • double aortic arch(MP:0004159)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)

  • embryonic lethality, complete penetrance(MP:0011092)

  • nervous system phenotype(MP:0003631)

  • right aortic arch(MP:0004158)

  • transposition of great arteries(MP:0004110)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, SV40 poly A, mouse C57BL/6 Neuropilin-1 genomic DNA
    Research applicationCardiovascular Research
    Developmental Biology Research
    Hematological Research
    Immunology and Inflammation Research
    Neurobiology Research
    covid19_infection
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 19, 995-1005 (1997).
    DepositorHajime Fujisawa (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Hatanaka Y, Matsumoto T, Yanagawa Y, Fujisawa H, Murakami F, Masu M.
    Distinct roles of neuropilin 1 signaling for radial and tangential extension of callosal axons.
    J Comp Neurol 514(3) 215-25(2009) 19296474