Strain Data Sheet

RBRC01088

Strain Information

Image
BRC No.RBRC01088
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Hras<tm1Mok>/MokRbrc
Former Common nameH-ras KO, B6-Hras KO, B6.129S-Hras1<tm1Mok>
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences (Institute of Medical Science, University of Tokyo).
Strain descriptionHras gene knockout mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Targeted deletion of the H-ras gene decreases tumor formation in mouse skin carcinogenesis.
Ise K, Nakamura K, Nakao K, Shimizu S, Harada H, Ichise T, Miyoshi J, Gondo Y, Ishikawa T, Aiba A, Katsuki M
Oncogene, 19, 2951-2956 (2000). 10871846

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Hras
MGI:96224		Harvey rat sarcoma virus oncogene7Hras<tm1Mok>
MGI:2386128		targeted mutation 1, Motoya Katsuki
  • Costello syndrome(DisGeNET, MedGEN)

  • large congenital melanocytic nevus(MedGEN)

  • linear nevus sebaceous syndrome(MedGEN)
    • more 4 Diseases
  • melanoma(DisGeNET)

  • nevus, epidermal(MedGEN)

  • thyroid cancer, nonmedullary, 2(MedGEN)

  • urinary bladder cancer(MedGEN)
    • neo neomycin resistance gene (E. coli)7 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • ascites(MP:0005324)

  • chylous ascites(MP:0012080)

  • decreased incidence of tumors by chemical induction(MP:0004502)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo gene, mouse H-ras genomic DNA
    Research applicationCancer Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Oncogene. 19, 2951-2956 (2000). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorToshikuni Sasaoka (Niigata University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data