Strain Data Sheet

RBRC01089

Strain Information

Image
BRC No.RBRC01089
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Nras<tm1Mok>
Former Common nameN-ras KO, B6-Nras KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strainC57BL/6
Appearance
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences (Institute of Medical Science, University of Tokyo).
Strain descriptionNras gene knockout mice. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
K-ras is essential for the development of the mouse embryo.
Koera K, Nakamura K, Nakao K, Miyoshi J, Toyoshima K, Hatta T, Otani H, Aiba A, Katsuki M
Oncogene, 15, 1151-1159 (1997). 9294608

K-Ras mediates cytokine-induced formation of E-cadherin-based adherens junctions during liver development.
Matsui T, Kinoshita T, Morikawa Y, Tohya K, Katsuki M, Ito Y, Kamiya A, Miyajima A
EMBO J. 21(5):1021-30 (2002). 11867530

Partial functional overlap of the three ras genes in mouse embryonic development.
Nakamura K, Ichise H, Nakao K, Hatta T, Otani H, Sakagami H, Kondo H, Katsuki M
Oncogene, 27, 2961-2968 (2008). 18059342

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Csde1
MGI:92356		cold shock domain containing E1, RNA binding3Csde1
Nras
MGI:97376		neuroblastoma ras oncogene3Nras<tm1Mok>
MGI:3794957		targeted mutation 1, Motoya Katsuki
  • autoimmune lymphoproliferative syndrome type 4(MedGEN)

  • colorectal cancer(MedGEN)

  • colorectal carcinoma(DisGeNET)
    • more 7 Diseases
  • juvenile myelomonocytic leukemia(DisGeNET)

  • large congenital melanocytic nevus(MedGEN)

  • linear nevus sebaceous syndrome(MedGEN)

  • neurocutaneous melanocytosis(MedGEN)

  • nevus, epidermal(MedGEN)

  • Noonan syndrome 6(MedGEN)

  • thyroid cancer, nonmedullary, 2(MedGEN)
    • neo neomycin resistance gene (E. coli)3 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • ascites(MP:0005324)

  • chylous ascites(MP:0012080)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E coli neo, mouse N-ras genomic DNA, mouse Csde1 genomic DNA
    Research applicationCancer Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 21, 1021-1030 (2002). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorToshikuni Sasaoka (Niigata University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data