Strain Data Sheet

RBRC01099

Strain Information

Image
BRC No.RBRC01099
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(CAG-XBP1*/venus)#Miur/MiurRbrc
Former Common nameER stress-activated indicator (ERAI), ERAI mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Takao Iwawaki and Masayuki Miura, RIKEN Brain Science Institute. The 3.6-kb fragment of pCAX-F-XBP1deltaDBD-venus was injected into the pronuclei of C57BL/6 fertilized eggs. Transgenic mice were backcrossed to C57BL/6JJcl.
Strain descriptionERAI (ER stress-activated indicator) transgenic mice carry human XBP1 and venus (a variant of green fluorescent protein) fusion gene under control of a CAG promoter. Endoplasmic reticulum (ER) stress is caused by the accumulation of unfolded proteins in the ER lumen, and is associated with vascular and neurodegenerative diseases. The transcripts from ERAI constructs are spliced under ER stress, the spliced mRNA is translated into an XBP1-venus fusion protein, which can be detected by fluorescence of the venus. This strain is useful to monitor physiological and pathological ER stress in vivo.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]. Tg mice are viable and fertile.
References
A transgenic mouse model for monitoring endoplasmic reticulum stress.
Iwawaki T, Akai R, Kohno K, Miura M
Nat. Med., 10, 98-102 (2004). 14702639

Health Report

Examination Date / Room / Rack2024/11/18Room:4-ARack:CSentinel mouse program
2024/08/19Room:4-ARack:CSentinel mouse program
2024/05/20Room:4-ARack:CSentinel mouse program
2024/02/19Room:4-ARack:CSentinel mouse program
2023/11/20Room:4-ARack:CSentinel mouse program
2023/11/13Room:4-ARack:CAdditional tests
2023/08/21Room:4-ARack:CSentinel mouse program
2023/08/07Room:4-ARack:CAdditional tests
2023/05/22Room:4-ARack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLAG FLAG tag (synthetic)UNFLAG CMV-IE enhancer, chicken beta-actin promoter
Venus Yellow Fluorescent Protein (Aequorea victoria)UNVenus
XBP1 X-box binding protein 1 (human)UNXBP1

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype dataPhenopub Link

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 intron and polyA signal, human XBP1 cDNA, Jellyfish venus (GFP) cDNA, FLAG tag
    Research applicationCell Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Med., 10, 98-102 (2004). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. During 2 years after deposition by the DEPOSITOR to the RIKEN BRC, the RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR, and to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE. The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR. The material shall be used for teaching or not-for-profit research purposes only for the research described within this MTA. If there is intent to use the material for profit purpose, the recipient shall first discuss its intentions with RIKEN. The recipient shall agree to acknowledge RIKEN in all publications with regard to the material. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorMasayuki Miura (The University of Tokyo)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Mar 2007

    BRC mice in Publications

    Norlin S, Parekh VS, Naredi P, Edlund H.
    Asna1/TRC40 Controls β-Cell Function and Endoplasmic Reticulum Homeostasis by Ensuring Retrograde Transport.
    Diabetes 65(1) 110-9(2016) 26438609
    Bhootada Y, Kotla P, Zolotukhin S, Gorbatyuk O, Bebok Z, Athar M, Gorbatyuk M.
    Limited ATF4 Expression in Degenerating Retinas with Ongoing ER Stress Promotes Photoreceptor Survival in a Mouse Model of Autosomal Dominant Retinitis Pigmentosa.
    PLoS One 11(5) e0154779(2016) 27144303
    Wang X, Wang G, Kunte M, Shinde V, Gorbatyuk M.
    Modulation of angiogenesis by genetic manipulation of ATF4 in mouse model of oxygen-induced retinopathy [corrected].
    Invest Ophthalmol Vis Sci 54(9) 5995-6002(2013) 23942974
    Kunte MM, Choudhury S, Manheim JF, Shinde VM, Miura M, Chiodo VA, Hauswirth WW, Gorbatyuk OS, Gorbatyuk MS.
    ER stress is involved in T17M rhodopsin-induced retinal degeneration.
    Invest Ophthalmol Vis Sci 53(7) 3792-800(2012) 22589437
    Cao L, Kobayakawa S, Yoshiki A, Abe K.
    High resolution intravital imaging of subcellular structures of mouse abdominal organs using a microstage device.
    PLoS One 7(3) e33876(2012) 22479464
    Martino ME, Olsen JC, Fulcher NB, Wolfgang MC, O'Neal WK, Ribeiro CM.
    Airway epithelial inflammation-induced endoplasmic reticulum Ca2+ store expansion is mediated by X-box binding protein-1.
    J Biol Chem 284(22) 14904-13(2009) 19321437
    Kamimura D, Bevan MJ.
    Endoplasmic reticulum stress regulator XBP-1 contributes to effector CD8+ T cell differentiation during acute infection.
    J Immunol 181(8) 5433-41(2008) 18832700
    Braun, M., Tatreau, J., Fulcher, N., Wolfgang, M., Randell, S., O’Neal, W., and Ribeiro, C. M. P.
    Pseudomonas aeruginosa activates the unfolded protein response in airway epithelia: relevance for airway ・ inflammation.
    Pediatric Pulmonology, Supplement 30 260(2007)