Strain Data Sheet

RBRC01144

Strain Information

Image
BRC No.RBRC01144
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S7-Heyl<tm1Kkb>/YsaRbrc
Former Common namehairy and enhancer of split-related 3 Knock in (LacZ)
H-2 Haplotype
ES Cell lineAB1 [129S7/SvEvBrd-Hprt<+>]
Background strainC57BL/6NJcl
Appearanceblack [a/a , B/B , C/C]
Strain developmentB6;129S7-Heyl<tm1Kkb>. The Heyl gene is a member of the hairy and enhancer of split related family of bHLH transcription factors, play important roles in mediating the Notch signaling pathway. It is suggested that the Heyl gene has a role of the differentiation of retina. This strain is useful for elucidation of the Notch-signaling pathway mechanism.
Strain descriptionDeveloped by Drs. H. Kokubo and R.L. Johnson at University of Texas M.D. Anderson Cancer Center in 1999. Combined loxP site and lacZ-Neo cassette was transferred into Heyl gene.
Colony maintenanceSibling mating.Homozygous mutant mice are fertile and display no obvious defects.
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Heyl
MGI:1860511		hairy/enhancer-of-split related with YRPW motif-like4Heyl<tm1Kkb>
MGI:5302514		targeted mutation 1, Hiroki Kokubo
lacZ beta-galactosidase (E. coli)4
loxP phage P1 loxP4loxP
loxP phage P1 loxP4loxP
neo neomycin resistance gene (E. coli)4

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal muscle regeneration(MP:0000750)

  • abnormal skeletal muscle morphology(MP:0000759)

  • abnormal skeletal muscle satellite cell proliferation(MP:0009410)

  • decreased body size(MP:0001265)

  • decreased body weight(MP:0001262)
    • more 7 phenotypes
  • decreased muscle weight(MP:0004232)

  • decreased satellite cell number(MP:0003833)

  • decreased skeletal muscle fiber number(MP:0009406)

  • decreased skeletal muscle fiber size(MP:0009400)

  • impaired skeletal muscle regeneration(MP:0013239)

  • muscle phenotype(MP:0005369)

  • postnatal lethality, incomplete penetrance(MP:0011086)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse hairy and enhancer of split-related 3 genomic DNA, E. coli lacZ, E. coli neo, phage P1 loxP
    Research application
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorYumiko Saga (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data