Strain Data Sheet

RBRC01147

Strain Information

Image
BRC No.RBRC01147
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Mesp2<tm4Ysa>/YsaRbrc
Former Common nameMesp2L
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainICR MCH
Appearance
Strain development
Strain description
Colony maintenance
References
Mesp2: a novel mouse gene expressed in the presegmented mesoderm and essential for segmentation initiation.
Saga Y, Hata N, Koseki H, Taketo M M
Genes Dev., 11, 1827-1839 (1997). 9242490

Mesp2 initiates somite segmentation through the Notch signalling pathway.
Takahashi Y, Koizumi K, Takagi A, Kitajima S, Inoue T, Koseki H, Saga Y
Nat. Genet., 25, 390-396 (2000). 10932180

Mouse Nkd1, a Wnt antagonist, exhibits oscillatory gene expression in the PSM under the control of Notch signaling.
Ishikawa A, Kitajima S, Takahashi Y, Kokubo H, Kanno J, Inoue T, Saga Y
Mech. Dev., 121, 1443-1453 (2004). 15511637

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mesp2
MGI:1096325		mesoderm posterior 27Mesp2<tm4Ysa>
MGI:2386844		targeted mutation 4, Yumiko Saga
  • spondylocostal dysostosis 1, autosomal recessive(MedGEN)

  • spondylocostal dysostosis 2, autosomal recessive(MedGEN)
    • lacZ beta-galactosidase (E. coli)7
    neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal somite development(MP:0001688)

  • abnormal vertebral lamina morphology(MP:0004605)

  • abnormal vertebral pedicle morphology(MP:0004604)

  • abnormal vertebral transverse process morphology(MP:0004600)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Mesp2 genomic DNA, E. coli lacZ, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
    Research applicationDevelopmental Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Genet., 25, 390-396 (2000).
    DepositorYumiko Saga (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data