Strain Data Sheet

RBRC01162

Strain Information

Image
BRC No.RBRC01162
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-EEA1/EGFP)12Tssk/TsskRbrc
Former Common nameEGFP-EEA1-12 transgenic mouse
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Sasaki (2002). The EGFP-EEA1 plasmid was injected into the pronucleous of C57BL/6JJcl fertilized eggs.
Strain descriptionC57BL/6J-Tg(CAG-EEA1/EGFP)12Tssk. This transgenic mouse expresses the GFP and EEA1 fusion gene under the control of the CAG promoter.
Colony maintenanceSibling Mating
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EEA1
MGI:2442192		Early endosome antigen 1UNEEA1
EGFP Green Fluorescent Protein (Aequorea victoria)UNEGFP CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), EEA1 (Early endosome antigen 1) cDNA, Jellyfish GFP cDNA
    Research applicationCell Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT is requested to submit the abstract of the research project to the DEPOSITOR, Dr. Sasaki T.
    DepositorTakehiko Sasaki (Akita University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data