Strain Data Sheet

RBRC01193

Strain Information

Image
BRC No.RBRC01193
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Lck-Ppp3ca*)28Naka/NakaRbrc
Former Common namecalcineurin dominant negative Tg mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Toshinori Nakayama, Graduate School of Medicine, Chiba University in 1999.
Strain descriptionThis transgenic mice express a dominant-negative type of calcineurin under the control of a proximal lck promoter.
Colony maintenance
References
T cell receptor-induced calcineurin activation regulates T helper type 2 cell development by modifying the interleukin 4 receptor signaling complex.
Yamashita M, Katsumata M, Iwashima M, Kimura M, Shimizu C, Kamata T, Shin T, Seki N, Suzuki S, Taniguchi M, Nakayama T
J. Exp. Med., 191, 1869-1879 (2000). 10839803

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ppp3ca
MGI:107164		protein phosphatase 3, catalytic subunit, alpha isoformUNPpp3ca mouse lck proximal promoter, human growth hormone poly A signal
  • arthrogryposis, cleft palate, craniosynostosis, and impaired intellectual development(MedGEN)

  • epileptic encephalopathy, infantile or early childhood, 1(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • decreased T-helper 2 cell number(MP:0008091)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse proximal lck promoter, mouse calcineurin cDNA, human Growth Hormone poly A signal
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 191, 1869-1879 (2000).
    DepositorToshinori Nakayama (Chiba University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data