Strain Data Sheet
RBRC01230
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC01230 |
| Type | Targeted Mutation and Chromosomal Aberration and F2 Hybrid |
| Species | Mus musculus |
| Strain name | B6;129-Foxh1<tm1Hmd>/HmdRbrc |
| Former Common name | B6;129-FoxH1flox/flox, FASTflox/flox, B6;129-FoxH1tm2Hh(B6;129-FoxH1flox/flox,FASTflox/flox)Knockout mice |
| H-2 Haplotype | |
| ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
| Background strain | |
| Appearance | white-bellied agouti [A<w>/? B/B C/C] black [a/a B/B C/C] |
| Strain development | Developed by Drs. Masamichi Yamamoto and Hiroshi Hamada at Osaka University in 2001. C57BL/6Cr and (129X1/SvJ x 129S1/Sv)F1-Kitl+ mixed background. |
| Strain description | The transcription factor Foxh1 (horkhead box H1) gene mediates signaling by transforming growth factor-β, Activin, and Nodal. Foxh1 protein interacts with phospho-Smad2 to regulate specific downstream targets of these signals. Mutation in Foxh1 results in impaired orientation of anterior-posterior axis and various defects of anterior streak and node. The flox allele of this strain was generated by Flp-mediated deletion of the neo cassette located between frt sites from the original targeting vector which was designed to insert a combined loxP site and loxP-frt-neo-frt cassette. Conditional Foxh1 deficient mice can be generated by crossing with tissue-specific Cre mice. This strain is useful for the study of Foxh1 gene in vivo. |
| Colony maintenance | Sibling Mating (Homozygote x Homozygote)Homozygote mutant mice are normal and fertile. |
| References | The transcription factor FoxH1 (FAST) mediates Nodal signaling during anterior-posterior patterning and node formation in the mouse. Yamamoto M, Meno C, Sakai Y, Shiratori H, Mochida K, Ikawa Y, Saijoh Y, Hamada H Genes Dev., 15, 1242-1256 (2001). 11358868 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| FoxH1 MGI:1347465 | forkhead box H1 | 15 | FoxH1<tm1Hmd> MGI:2387775 | targeted mutation 1, Hiroshi Hamada | |||
| Frt | yeast FRT (flippase recombination target) site | 15 | Frt | ||||
| loxP | phage P1 loxP | 15 | loxP | ||||
| loxP | phage P1 loxP | 15 | loxP | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse FoxH1 genomic DNA |
| Research application | Cre/loxP system Developmental Biology Research |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev., 15, 1242-1256 (2001). |
| Depositor | Hiroshi Hamada (RIKEN CDB) |
| Strain Status |  Frozen embryos |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |