Strain Data Sheet

RBRC01253

Strain Information

Image
BRC No.RBRC01253
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Gopc<tm1Tno>/Rbrc
Former Common nameGOPC Knockout mouse
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Drs. Ryoji Yao and Tetsuo Noda, Japanese Foundation for Cancer Research Cancer Institute in 1999. Genomic sequence containing 2, 2b, 3 and 4 of Gopc was replaced by the insertion of a LacZ and a floxed neo cassette. This strain originated on a B6;129 background.
Strain descriptionB6;129S4-Gopc<tm1Tno>. Gopc, golgi associtated PDZ and coiled-coil motif containing that predominantly localized at trans-Golgi region, propose that play a role in vesicle transport from the Golgi. The Gopc deficient male mice are infertile that spermatozoa showed complete lack of acrosomes. This knockout mice are proved to be powerful tools for understanding the mechanisms underlying spermatogenesism, and valuable animal mode for human globozoospermia.
Colony maintenanceSibling Mating (Heterozygote x Wild-type, Heterozygote)
References
Lack of acrosome formation in mice lacking a Golgi protein, GOPC.
Yao R, Ito C, Natsume Y, Sugitani Y, Yamanaka H, Kuretake S, Yanagida K, Sato A, Toshimori K, Noda T
Proc. Natl. Acad. Sci. USA, 99, 11211-11216 (2002). 12149515

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gopc
MGI:2149946		golgi associated PDZ and coiled-coil motif containing10Gopc<tm1Tno> targeted mutation 1, Tetsuo Noda
lacZ beta-galactosidase (E. coli)10
loxP phage P1 loxP10loxP
loxP phage P1 loxP10loxP
neo neomycin resistance gene (E. coli)10 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
nls SV40 large T antigen nuclear localization signal (NLS)10nls
SV40 polyA10

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal acrosome morphology(MP:0008898)

  • abnormal sperm flagellum morphology(MP:0008892)

  • abnormal sperm mitochondrial sheath morphology(MP:0009832)

  • abnormal sperm nucleus morphology(MP:0009232)

  • abnormal spermatogenesis(MP:0001156)
    • more 7 phenotypes
  • absent acrosome(MP:0008839)

  • asthenozoospermia(MP:0002675)

  • coiled sperm flagellum(MP:0009238)

  • detached acrosome(MP:0009231)

  • globozoospermia(MP:0002686)

  • impaired fertilization(MP:0000242)

  • male infertility(MP:0001925)
    • Detailed phenotype data

    Ordering Information

    Donor DNASimian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, SV40 polyA, phage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse GOPC genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 99, 11211-11216 (2002). It is agreed by the parties hereto that any patent or other intellectual property right to any inventions to be made by or derived of the use by the RECIPIENT of the BIOLOGICAL RESOURCE shall be jointly owned by the RECIPIENT and the DEPOSITOR. The share in the ownership on such patents etc. shall be agreed between the RECIPIENT and the DEPOSITOR. In the event that the RECIPIENT desires to file patent applications for such inventions, the RECIPIENT shall obtain a prior written consent of the DEVELOPER, Dr. Tetsuo Noda before the RECIPIENT files such patent applications.
    The RECIPIENT should negotiate in good faith with the DEPOSITOR, or its representatives, to agree a fair and reasonable consideration to the DEPOSITOR in respect of the contribution to the said intellectual property rights afforded by the provision of the BIOLOGICAL RESOURCE hereunder.
    The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
    The RECIPIENT agrees to include the DEVELOPER, Dr. Tetsuo Noda as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
    DepositorTetsuo Noda (Japanese Foundation for Cancer Research)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Bizkarguenaga M, Gomez-Santos L, Madrid JF, Sáez FJ, Alonso E.
    Zona Pellucida sperm-binding protein 3 receptor distribution during Gopc-/- globozoospermic spermatogenesis.
    Microsc Res Tech 85(4) 1454-1464(2022) 34870349