Strain Data Sheet

RBRC01254

Strain Information

Image
BRC No.RBRC01254
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Cnot7<tm1Tno>/Rbrc
Former Common nameCnot7 Knockout mouse
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Ryoji Yao, Tadashi Yamamoto, and Tetsuo Noda, Japanese Foundation for Cancer Research Cancer Institute in 1999. An exon of Cnot7 subcloned the 18-kb genomic DNA fragment, was replaced by the insertion of a LacZ and a floxed neo cassette. The floxed neo cassette was excised in this strain. This strain originated on a B6;129 background.
Strain descriptionB6;129S4-Cnot7<tm1Tno>. Cnot7 gene (CCR4-NOT transcription complex. subunit 7) is essential for spermatogenesis. Cnot7 deficient male mice are sterile owing to oligo-astheno-teratozoospermia, impaired sperm motility, unsynchronized spermatid maturation, and Sertoli cell abnormalities. This knockout mouse strain is a powerful tool for understanding the mechanisms underlying spermatogenesis.
Colony maintenanceHeterozygote x Wild-typeHomozygous mutant males are sterile.
References
Oligo-astheno-teratozoospermia in mice lacking Cnot7, a regulator of retinoid X receptor beta.
Nakamura T, Yao R, Ogawa T, Suzuki T, Ito C, Tsunekawa N, Inoue K, Ajima R, Miyasaka T, Yoshida Y, Ogura A, Toshimori K, Noce T, Yamamoto T, Noda T
Nat. Genet., 36, 528-533 (2004). 15107851

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cnot7
MGI:1298230		CCR4-NOT transcription complex, subunit 78Cnot7<tm1Tno>
MGI:3044887		targeted mutation 1, Tetsuo Noda
lacZ beta-galactosidase (E. coli)8
loxP phage P1 loxP8loxP
loxP phage P1 loxP8loxP
neo neomycin resistance gene (E. coli)8 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
nls SV40 large T antigen nuclear localization signal (NLS)8nls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal Sertoli cell morphology(MP:0002784)

  • abnormal male germ cell apoptosis(MP:0008280)

  • abnormal male germ cell morphology(MP:0006362)

  • abnormal sperm flagellum morphology(MP:0008892)

  • abnormal sperm head morphology(MP:0009230)
    • more 9 phenotypes
  • abnormal sperm mitochondrial sheath morphology(MP:0009832)

  • abnormal spermatid morphology(MP:0006380)

  • asthenozoospermia(MP:0002675)

  • male infertility(MP:0001925)

  • multinucleated giant male germ cells(MP:0020550)

  • oligozoospermia(MP:0002687)

  • seminiferous tubule degeneration(MP:0001154)

  • small testis(MP:0001147)

  • teratozoospermia(MP:0005578)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli neo, Simian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, mouse Cnot-7 genomic DNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), Phage P1 loxP
    Research applicationCell Biology Research
    Genetics Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Genetics, 36, 528-533 (2004). It is agreed by the parties hereto that any patent or other intellectual property right to any inventions to be made by or derived of the use by the RECIPIENT of the BIOLOGICAL RESOURCE shall be jointly owned by the RECIPIENT and the DEPOSITOR. The share in the ownership on such patents etc. shall be agreed between the RECIPIENT and the DEPOSITOR. In the event that the RECIPIENT desires to file patent applications for such inventions, the RECIPIENT shall obtain a prior written consent of the DEVELOPER, Dr. Tetsuo Noda before the RECIPIENT files such patent applications.
    The RECIPIENT should negotiate in good faith with the DEPOSITOR, or its representatives, to agree a fair and reasonable consideration to the DEPOSITOR in respect of the contribution to the said intellectual property rights afforded by the provision of the BIOLOGICAL RESOURCE hereunder.
    The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
    The RECIPIENT agrees to include the DEVELOPER, Dr. Tetsuo Noda as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
    DepositorTetsuo Noda (Japanese Foundation for Cancer Research)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data