Strain Data Sheet

RBRC01260

Strain Information

Image
BRC No.RBRC01260
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129(Cg)-Xist<tm2Sado>
Former Common nameXist1loxGFP mice
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Takashi Sado, National Institute of Genetics at 2004. R1 ES cells were used to generate the mutant mice. The mice were crossed to C57BL/6.
Strain descriptionXist gene knockout mice (Xist<1loxGFP>). Heterozygote mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).
Colony maintenanceHeterozygote (female) x Wild-type (male) [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)X
IRES internal ribosomal entry site (EMCV)X
SA Splice acceptorXSA
Xist
MGI:98974		inactive X specific transcriptsXXist<tm2Sado>
MGI:3583750		targeted mutation 2, Takashi Sado
loxP phage P1 loxPXloxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • cellular phenotype(MP:0005384)
    • Detailed phenotype data

    Ordering Information

    Donor DNASA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), jellyfish GFP cDNA, poly A, phage P1 loxP site, mouse Xist genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must inform the DEPOSITOR the research project using the
    BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to
    avoid the conflict of interest with the DEPOSITOR.
    The RECIPIENT should contact the DEPOSITOR in the case of application for
    any patents with the results from these mice.
    DepositorTakashi Sado (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Feb 2009

    BRC mice in Publications

    Hirasawa R, Matoba S, Inoue K, Ogura A.
    Somatic donor cell type correlates with embryonic, but not extra-embryonic, gene expression in postimplantation cloned embryos.
    PLoS One 8(10) e76422(2013) 24146866
    Inoue K, Kohda T, Sugimoto M, Sado T, Ogonuki N, Matoba S, Shiura H, Ikeda R, Mochida K, Fujii T, Sawai K, Otte AP, Tian XC, Yang X, Ishino F, Abe K, Ogura A.
    Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer.
    Science 330(6003) 496-9(2010) 20847234
    Inoue K, Ogonuki N, Mekada K, Yoshiki A, Sado T, Ogura A.
    Sex-reversed somatic cell cloning in the mouse.
    J Reprod Dev 55(5) 566-9(2009) 19602850