Strain Data Sheet
RBRC01267
Strain Information | |
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| Image |  |
| BRC No. | RBRC01267 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | 129(Cg)-Numb<tm1Zili/tm1.1Zili> Numbl<tm1Zili/tm1.1Zili>/SiecRbrc |
| Former Common name | Numb [-/loxP]; Numblike [-/FRTneoRFRTloxP] |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Targeting of Numb: 1997, Olav Zilian, ISRECTargeting of Numblike: 2001,Olav Zilian, ISREC |
| Strain description | Compound heterozygous mice conditionally targeted for Numb and Numblike (loxP) and some of them deleted for the other conditional allele. The selection marker in Numb (loxPneoRloxP) was deleted by Cre recombinase. However, this cassette is still present in the conditionally targeted allele of Numblike in these mice as a cassette flanked with FRT sites (FRTneoRFRT; [loxn eo]). In general, the purpose of the conditional alleles is to analyze the function of the Numb and Numblike proteins in mice after their conditional ablation upon expression of Cre recombinase. Parents heterozygous for a deleted allele of each gene allow to generate (1) compound homozygous null mutant embryos for the study of embryonic phenotypes or (2) adult mice homozygous for the deletion of Numblike. |
| Colony maintenance | Compound heterozygous mice are efficient breeders (Numb[loxP / -]; Numblike[loxP / -]). By contrast, mice homozygous for the deletion of Numblike have a small litter size and, hence, reproduce inefficiently. That problem of low breeding efficiency might rely on diminished fertility of he female homozygotes. |
| References | Multiple roles of mouse Numb in tuning developmental cell fates. Zilian O, Saner C, Hagedorn L, Lee H Y, Sauberli E, Suter U, Sommer L, Aguet M Curr. Biol., 11, 494-501 (2001). 11412999Normal hemopoiesis and lymphopoiesis in the combined absence of numb and numblike. Wilson A, Ardiet D L, Saner C, Vilain N, Beermann F, Aguet M, Macdonald H R, Zilian O J. Immunol., 178(11):6746-6751 (2007). 17513721 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Numb MGI:107423 | NUMB endocytic adaptor protein | 12 | Numb<tm1.1Zili> | targeted mutation 1.1, Olav Zilian | |||
| loxP | phage P1 loxP | 12 | loxP | ||||
| loxP | phage P1 loxP | 12 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 12 | |||||
| Frt | yeast FRT (flippase recombination target) site | 7 | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | 7 | Frt | ||||
| Numbl MGI:894702 | numb-like | 7 | Numbl | targeted mutation 1.1, Olav Zilian | |||
| neo | neomycin resistance gene (E. coli) | 7 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | more 29 phenotypes |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | yeast FRT (flipase recombination target) sites, phage P1 loxP site, E. coli neo, mouse Numb genomic DNA, mouse Numblike genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter) |
| Research application | Cell Biology Research Cre/loxP system |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Any publication by the DEPOSITOR describing the BIOLOGICAL RESOURCE for the first time. (For targeted Numb: Olav Zillan et al. Current Biology. 11(7): 494-501; for targeted Numblike : the first publication describing this allele by the DEPOSITOR). This term is to be respected in particular when the first 2 years after deposition of the BIOLOGICAL RESOURCE are passed.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. This term is to be respected in particular when the material was requested after the first 2 years upon deposition of the BIOLOGICAL RESOURCE.1) The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. Furthermore, the RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE in research projects initiated during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. 2) Further, the RECIPIENT must inform the DEPOSITOR about the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR. 3) Furthermore, prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. |
| Depositor | Olav Zilian (Institut Suisse de Recherche Experimentale sur le Cancer (ISREC)) |
| Strain Status |  Frozen embryos |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |