Strain Data Sheet

RBRC01268

Strain Information

Image
BRC No.RBRC01268
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Numb<tm1Zili> Numbl<tm1Zili>/SiecRbrc
Former Common nameNumb [loxP/loxP]; Numblike [loxP/loxP]
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentTargeting of Numb: 1997, Olav Zilian, ISRECTargeting of Numblike: 2001,Olav Zilian, ISREC
Strain descriptionCompound mice conditionally targeted for Numb and Numblike (loxP; for nomenclature of the alleles: lox) ; the selection marker in Numb (loxPneoRloxP) and Numblike (FRTneoRFRT) was deleted by Cre and Flp recombinase, respectively. The mice ready for shipment to RIKEN BRC carry at this point the conditional alleles over a non-targeted, wild-type allele [lox/+]; absence of the transgene FLPe should be reconfirmed in their progeny.Purpose of these conditional alleles: functional analysis of the Numb and Numblike proteins in mice by their conditional ablaion through expression of Cre recombinase.
Colony maintenanceEfficient breeding is expected for these compound mice once homozygous for each conditionally targeted allele (Numb[lox / lox]; Numblike[lox / lox]) as they were efficient breeders when the compound homozygotes still carried FRTneoRFRT in both Numblike lox alleles.
References
Multiple roles of mouse Numb in tuning developmental cell fates.
Zilian O, Saner C, Hagedorn L, Lee H Y, Sauberli E, Suter U, Sommer L, Aguet M
Curr. Biol., 11, 494-501 (2001). 11412999

Normal hemopoiesis and lymphopoiesis in the combined absence of numb and numblike.
Wilson A, Ardiet D L, Saner C, Vilain N, Beermann F, Aguet M, Macdonald H R, Zilian O
J. Immunol., 178(11):6746-6751 (2007). 17513721

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Numb
MGI:107423		NUMB endocytic adaptor protein12Numb<tm1Zili> targeted mutation 1, Olav Zilian
loxP phage P1 loxP12loxP
loxP phage P1 loxP12loxP
Frt yeast FRT (flippase recombination target) site7Frt
Numbl
MGI:894702		numb-like7Numbl<tm1Zili> targeted mutation 1, Olav Zilian

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal Purkinje cell dendrite morphology(MP:0008572)

  • abnormal cerebellar Purkinje cell layer(MP:0000875)

  • abnormal cerebellar granule layer morphology(MP:0000886)

  • abnormal cerebellar lobule formation(MP:0006097)

  • abnormal cerebellum development(MP:0000854)
    • more 10 phenotypes
  • abnormal cerebellum external granule cell layer morphology(MP:0000872)

  • abnormal cerebellum vermis morphology(MP:0000864)

  • abnormal intestinal cholesterol absorption(MP:0002645)

  • ataxia(MP:0001393)

  • decreased Purkinje cell number(MP:0000880)

  • decreased circulating cholesterol level(MP:0005179)

  • hematopoietic system phenotype(MP:0005397)

  • postnatal lethality, incomplete penetrance(MP:0011086)

  • small cerebellum(MP:0000852)

  • thin cerebellar molecular layer(MP:0000890)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP, mouse Numb genomic DNA, mouse Numblike genomic DNA, yeast FRT (flipase recombination target) site
    Research applicationCell Biology Research
    Cre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Any publication by the DEPOSITOR describing the BIOLOGICAL RESOURCE for the first time. (For targeted Numb: Olav Zillan et al. Current Biology. 11(7): 494-501; for targeted Numblike : the first publication describing this allele by the DEPOSITOR). This term is to be respected in particular when the first 2 years after deposition of the BIOLOGICAL RESOURCE are passed.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. This term is to be respected in particular when the material was requested after the first 2 years upon deposition of the BIOLOGICAL RESOURCE.1) The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. Furthermore, the RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE in research projects initiated during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
    2) Further, the RECIPIENT must inform the DEPOSITOR about the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
    3) Furthermore, prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application.
    DepositorOlav Zilian (Institut Suisse de Recherche Experimentale sur le Cancer (ISREC))
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data