Strain Data Sheet

RBRC01270

Strain Information

Image
BRC No.RBRC01270
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Gnptab<tm1Zili>/SiecRbrc
Former Common nameStealth [lacZloxPneoRloxP/+]
H-2 Haplotype
ES Cell line
Background strain129
Appearance
Strain developmentTargeting of Stealth: 2001, Denis-Luc Ardiet, ISREC
Strain descriptionMice with a lacZ knock-in in the first major coding exon of Stealth. The lacZ ORF begins with the first start codon of that stealth exon. Purpose of that mouse strain is to analyze stealth function in mice, in particular for its role in development, innate immunity, and homeostasis of organ systems.PGKneoR cassette flanked with loxP sites still present in this stealth null allele because these mice haven't been crossed with a deleter strain since there hasn't been one isogenic for 129SvEv(Allan Bradley).
Colony maintenanceThis strain has been kept isogenic for the genetic background 129SvEv (source: Allan Bradley) that is the very same genetic background of the ES cells in which the Stealth null allele was generated by a knock-in of lacZ in frame wih the major first start codon of Stealth. Hence, this precious isogenic line shall be kept breeding in its literally original background. In particular, the Stealth null allele is homozygous viable when isogenic for 129SvEv. However, such homozygotes don't breed, become sick with age and develop so far uncharacterized inflammatory processes and cachexia.
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gnptab
MGI:3643902		N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits10Gnptab<tm1Zili> targeted mutation 1, Olav Zilian
  • mucolipidosis type II(DisGeNET, MedGEN)

  • mucolipidosis type III, alpha/beta(MedGEN)
    • lacZ beta-galactosidase (E. coli)10
    loxP phage P1 loxP10loxP
    loxP phage P1 loxP10loxP
    neo neomycin resistance gene (E. coli)10

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • lethality at weaning, complete penetrance(MP:0011083)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli lacZ, E. coli neo, mouse stealth genomic DNA, SV40-3' UTR, phage P1 loxP
    Research applicationCell Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. To be cited is any publication by the DEPOSITOR describing the BIOLOGICAL RESOURCE for the first time. This term is to be respected in particular when the first 2 years after deposition of the BIOLOGICAL RESOURCE are passed.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. This term is to be respected in particular when the material was requested after the first 2 years upon deposition of the BIOLOGICAL RESOURCE.1) The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. Furthermore, the RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE in research projects initiated during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
    2) Further, the RECIPIENT must inform the DEPOSITOR about the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
    3)Furthermore, prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application.
    DepositorOlav Zilian (Institut Suisse de Recherche Experimentale sur le Cancer (ISREC))
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data