Strain Information | |
---|---|
Image | |
BRC No. | RBRC01297 |
Type | Transgene![]() |
Species | Mus musculus |
Strain name | B6.Cg-Tg(DBH-cre)6-8Koba/KobaRbrc |
Former Common name | DBH-Cre/6-8 |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J. |
Strain description | These transgenic mice express Cre recombinase gene under the control of the human dopamin beta hydroxylase (DBH) gene promoter. This strain can be used to make an inducible knock-out by crossing with the other genetically-engineered mice that have a loxP site-flanked sequence of interest. The recombination occurs in typical noradrenergic and adrenergic cell groups as well as in transient catecholaminergic cell types. Line 6-8 (RBRC01297), Line 9-9 (RBRC01492). |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
References | Fate of transient catecholaminergic cell types revealed by site-specific recombination in transgenic mice. Matsushita N, Kobayashi K, Miyazaki J, Kobayashi K J. Neurosci. Res., 78, 7-15 (2004). 15372500 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |||||||
---|---|---|---|---|---|---|---|
Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
cre | Phage P1 Cre recombinase | UN | cre | human dopamine beta-hydroxylase (DBH) promoter, rabbit beta-globin intron, rabbit beta-globin poly A, SV40 early gene poly A |
Phenotype | |
---|---|
Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data | ![]() |
Ordering Information | |
---|---|
Donor DNA | human dopamine beta-hydroxylase (DBH) promoter, rabbit beta-globin intron, phage P1 Cre recombinase gene, rabbit beta-globin poly A, SV40 early gene poly A |
Research application | Cell Biology Research Cre/loxP system Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neursci. Res., 78, 7-15 (2004). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Kazuto Kobayashi (Fukushima Medical University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Jun 2005 Mouse of the Month June 2006 |
BRC mice in Publications |
---|
Nakamura A, Muroi Y, Ishii T. Locus Coeruleus-Noradrenergic Neurons Regulate Stress Coping During Subchronic Exposure to Social Threats: A Characteristic Feature in Postpartum Female Mice. Cell Mol Neurobiol 43(5) 2359-2376(2023) 36577871 |