Strain Data Sheet

RBRC01297

Strain Information

Image
BRC No.RBRC01297
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(DBH-cre)6-8Koba/KobaRbrc
Former Common nameDBH-Cre/6-8
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J.
Strain descriptionThese transgenic mice express Cre recombinase gene under the control of the human dopamin beta hydroxylase (DBH) gene promoter. This strain can be used to make an inducible knock-out by crossing with the other genetically-engineered mice that have a loxP site-flanked sequence of interest. The recombination occurs in typical noradrenergic and adrenergic cell groups as well as in transient catecholaminergic cell types. Line 6-8 (RBRC01297), Line 9-9 (RBRC01492).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Fate of transient catecholaminergic cell types revealed by site-specific recombination in transgenic mice.
Matsushita N, Kobayashi K, Miyazaki J, Kobayashi K
J. Neurosci. Res., 78, 7-15 (2004). 15372500

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
cre Phage P1 Cre recombinaseUNcre human dopamine beta-hydroxylase (DBH) promoter, rabbit beta-globin intron, rabbit beta-globin poly A, SV40 early gene poly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
  • Detailed phenotype dataPhenopub Link

    Ordering Information

    Donor DNAhuman dopamine beta-hydroxylase (DBH) promoter, rabbit beta-globin intron, phage P1 Cre recombinase gene, rabbit beta-globin poly A, SV40 early gene poly A
    Research applicationCell Biology Research
    Cre/loxP system
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neursci. Res., 78, 7-15 (2004). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorKazuto Kobayashi (Fukushima Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Jun 2005
    Mouse of the Month June 2006

    BRC mice in Publications

    Nakamura A, Muroi Y, Ishii T.
    Locus Coeruleus-Noradrenergic Neurons Regulate Stress Coping During Subchronic Exposure to Social Threats: A Characteristic Feature in Postpartum Female Mice.
    Cell Mol Neurobiol 43(5) 2359-2376(2023) 36577871