Strain Data Sheet

RBRC01300

Strain Information

Image
BRC No.RBRC01300
TypeSpontaneous Mutation
SpeciesMus musculus
Strain nameD2.Cg-Prkdc<scid>/Rbrc
Former Common nameDBA/2-scid/+, DBA/2-scid mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Mie University Graduate School of Medicine. The original scid mice were provided by Ohtura Niwa, Radiation Biology Center, Kyoto University (C57BL/6-scid p<un>). The mutant mice were backcrossed to DBA/2Cr and C57BL/6NCr (RBRC01300 and RBRC01346, respectively).
Strain descriptionscid, severe combined immune deficiency mice. Homozygous mutant mice lack both functional T and B cells, resulting in severe combined immunodeficiency. DBA/2 background (RBRC01300), C57BL/6 background (RBRC01346). We do not carry out a detection of leaky mice by measuring serum Ig level (PCR genotying of scid mutation only).
Colony maintenanceHomozygote x Homozygote [or Crossing to DBA/2CrSlc]
References
Nonsense mutation at Tyr-4046 in the DNA-dependent protein kinase catalytic subunit of severe combined immune deficiency mice.
Araki R, Fujimori A, Hamatani K, Mita K, Saito T, Mori M, Fukumura R, Morimyo M, Muto M, Itoh M, Tatsumi K, Abe M
Proc. Natl. Acad. Sci. USA, 94, 2438-2443 (1997). 9122213

Persistent induction of somatic reversions of the pink-eyed unstable mutation in F1 mice born to fathers irradiated at the spermatozoa stage.
Shiraishi K, Shimura T, Taga M, Uematsu N, Gondo Y, Ohtaki M, Kominami R, Niwa O
Radiat. Res., 157, 661-667(2002). 12005545

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Prkdc
MGI:104779		protein kinase, DNA activated, catalytic polypeptide16Prkdc<scid> severe combined immune deficiency
  • severe combined immunodeficiency due to DNA-PKcs deficiency(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal CD4-positive, alpha beta T cell morphology(MP:0002432)

  • abnormal CD8-positive, alpha beta T cell morphology(MP:0005010)

  • abnormal NK cell physiology(MP:0010766)

  • abnormal Peyer's patch morphology(MP:0000696)

  • abnormal T cell differentiation(MP:0002145)
    • more 70 phenotypes
  • abnormal complement pathway(MP:0002471)

  • abnormal double-positive T cell morphology(MP:0002408)

  • abnormal humoral immune response(MP:0001800)

  • abnormal intestinal epithelium morphology(MP:0000488)

  • abnormal level of surface class II molecules(MP:0005042)

  • abnormal lymph node B cell domain morphology(MP:0002344)

  • abnormal lymph node morphology(MP:0002339)

  • abnormal pancreatic islet morphology(MP:0005215)

  • abnormal professional antigen presenting cell morphology(MP:0008195)

  • abnormal response to transplant(MP:0005671)

  • abnormal single-positive T cell number(MP:0008081)

  • abnormal spleen white pulp morphology(MP:0002357)

  • abnormal splenocyte morphology(MP:0009332)

  • abnormal thymus cortex morphology(MP:0002371)

  • abnormal thymus corticomedullary boundary morphology(MP:0009543)

  • abnormal thymus lobule morphology(MP:0002367)

  • abnormal thymus morphology(MP:0000703)

  • absent B cells(MP:0008071)

  • absent Peyer's patches(MP:0002831)

  • absent T cells(MP:0008070)

  • absent pre-B cells(MP:0000238)

  • absent thymus cortex(MP:0010250)

  • decreased B cell number(MP:0005017)

  • decreased B cell proliferation(MP:0005093)

  • decreased CD4-positive, alpha-beta T cell number(MP:0008075)

  • decreased CD8-positive, alpha-beta T cell number(MP:0008079)

  • decreased IgG level(MP:0001805)

  • decreased NK cell number(MP:0008045)

  • decreased T cell number(MP:0005018)

  • decreased T cell proliferation(MP:0005095)

  • decreased bone marrow cell number(MP:0000333)

  • decreased circulating insulin level(MP:0002727)

  • decreased erythrocyte cell number(MP:0002875)

  • decreased immunoglobulin level(MP:0002460)

  • decreased leukocyte cell number(MP:0000221)

  • decreased level of surface class I molecules(MP:0001841)

  • decreased lymphocyte cell number(MP:0005016)

  • decreased pancreatic beta cell number(MP:0003339)

  • decreased pre-B cell number(MP:0008209)

  • decreased susceptibility to autoimmune diabetes(MP:0004804)

  • decreased susceptibility to graft versus host disease(MP:0005673)

  • extended life span(MP:0001661)

  • hematopoietic system phenotype(MP:0005397)

  • homeostasis/metabolism phenotype(MP:0005376)

  • hyperglycemia(MP:0001559)

  • immune system phenotype(MP:0005387)

  • impaired complement classical pathway(MP:0002473)

  • impaired natural killer cell mediated cytotoxicity(MP:0005070)

  • increased NK cell number(MP:0008044)

  • increased T cell derived lymphoma incidence(MP:0002024)

  • increased cellular sensitivity to X-ray irradiation(MP:0002879)

  • increased circulating glucose level(MP:0005559)

  • increased eosinophil cell number(MP:0005011)

  • increased granulocyte number(MP:0000322)

  • increased length of allograft survival(MP:0004751)

  • increased macrophage cell number(MP:0005425)

  • increased mean corpuscular volume(MP:0002590)

  • increased monocyte cell number(MP:0000220)

  • increased neutrophil cell number(MP:0000219)

  • lymph node hypoplasia(MP:0008101)

  • lymphoid hypoplasia(MP:0002223)

  • muscle phenotype(MP:0005369)

  • premature death(MP:0002083)

  • salivary gland inflammation(MP:0001870)

  • small Peyer's patches(MP:0008135)

  • small lymphoid organs(MP:0000687)

  • small thymus(MP:0000706)

  • spleen hypoplasia(MP:0000694)

  • stomach inflammation(MP:0001873)

  • thymus cyst(MP:0014130)
    • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for basic research, shall not use for commercial use.
    DepositorKagemasa Kuribayashi (Mie University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data