Strain Data Sheet

RBRC01302

Strain Information

Image
BRC No.RBRC01302
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Srr2-Bgeo)1Okud/OkudRbrc
Former Common nameSRR2-βgeo transgenic mice
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Satoru Miyagi, Saitama Medical Scool at 2004. The transgene prasmid was injected into fertilized eggs. The transgenic mice were backcrossed to C57BL/6J.
Strain descriptionThese transgenic mice express lacZ in the ventricular layer of telencephalon in the embryo. This strain is used to trace neural stem cell/neural progenitor in vivo by X-gal staining.
Colony maintenance
References
The Sox-2 regulatory regions display their activities in two distinct types of multipotent stem cells.
Miyagi S, Saito T, Mizutani K, Masuyama N, Gotoh Y, Iwama A, Nakauchi H, Masui S, Niwa H, Nishimoto M, Muramatsu M, Okuda A
Mol. Cell. Biol., 24, 4207-4220 (2004). 15121842

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sox2 SRY (sex determining region Y)-box 2UNSox2
lacZ beta-galactosidase (E. coli)UN herpes simplex virus thymidine kinase promoter (HSV tk promoter)
neo neomycin resistance gene (E. coli)UN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse SRR2 genomic DNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), beta-geo (E. coli neo, E. coli lacZ), SV40 intron plus polyA addition signal from pGL2 luciferase vector
    Research applicationCell Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorAkihiko Okuda (Saitama Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data