Strain Data Sheet

RBRC01306

Strain Information

Image
BRC No.RBRC01306
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hbegf<tm1.1Mek>/MekRbrc
Former Common nameHB-EGF Knockout mice(BL6)(HBdel#80/B6)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6J
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs Ryo Iwamoto, Eisuke Mekada et al., at Osaka University in 2003.The HB-EGF null mice were generated by crossing with CAG-Cre transgenic mice.
Strain descriptionHbegf, heparin-binding EGF like growth factor is a member of the EGF family of growth factor. This protein binds to and activates the EGF receptor. Homozygous mutant mice exhibit grossly enlarged cardiac valves and ventricular chambers. Most homozygous mice died in the first postnatal week. LacZ gene is expressed under the native HB-EGF promoter to examine the expression pattern of the HB-EGF histologically.
Colony maintenance
References
Heparin-binding EGF-like growth factor and ErbB signaling is essential for heart function.
Iwamoto R, Yamazaki S, Asakura M, Takashima S, Hasuwa H, Miyado K, Adachi S, Kitakaze M, Hashimoto K, Raab G, Nanba D, Higashiyama S, Hori M, Klagsbrun M, Mekada E
Proc. Natl. Acad. Sci. USA, 100, 3221-3226 (2003). 12621152

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Hbegf
MGI:96070
heparin-binding EGF-like growth factor18Hbegf<tm1.1Mek>
MGI:2655703
targeted mutation 1.1, Eisuke Mekada
lacZ beta-galactosidase (E. coli)18
loxP phage P1 loxP18loxP
neo neomycin resistance gene (E. coli)18 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal aortic valve morphology(MP:0002747)

  • abnormal atrioventricular valve morphology(MP:0002745)

  • abnormal cardiovascular system physiology(MP:0001544)

  • abnormal heart ventricle outflow tract morphology(MP:0010224)

  • abnormal mitral valve morphology(MP:0000286)
  • more 11 phenotypes
  • abnormal pulmonary valve morphology(MP:0002748)

  • abnormal semilunar valve morphology(MP:0002746)

  • abnormal tricuspid valve morphology(MP:0002624)

  • decreased heart ventricle muscle contractility(MP:0005598)

  • dilated heart left ventricle(MP:0002753)

  • dilated heart right ventricle(MP:0002754)

  • dilated heart ventricle(MP:0008022)

  • enlarged heart(MP:0000274)

  • increased myocardial fiber size(MP:0004564)

  • postnatal lethality, incomplete penetrance(MP:0011086)

  • premature death(MP:0002083)
  • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP site, E. coli lacZ, poly A, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, poly A, mouse heparin-binding epidermal growth factor-like growth factor genomic DNA
    Research applicationCardiovascular Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
    DepositorEisuke Mekada (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Apr 2006

    BRC mice in Publications

    No Data