Strain Information | |
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Image | |
BRC No. | RBRC01307 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | C.Cg-Hbegf<tm1.1Mek>/MekRbrc |
Former Common name | HB-EGF Knockout mice(BALB) (HBdel#80/BALB) |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | BALB/cCrSlc |
Appearance | albino [A/A b/b c/c] |
Strain development | Developed by Drs Ryo Iwamoto, Eisuke Mekada et al., at Osaka University in 2003.The HB-EGF null mice were generated by crossing with CAG-Cre transgenic mice. |
Strain description | Hbegf, heparin-binding EGF like growth factor is a member of the EGF family of growth factor. This protein binds to and activates the EGF receptor. Homozygous mutant mice exhibit grossly enlarged cardiac valves and ventricular chambers. Most homozygous mutant mice die by 3 weeks of age. LacZ gene is expressed under the native HB-EGF promoter to examine the expression pattern of the HB-EGF histologically. |
Colony maintenance | Backcross to BALB/c (Heterozygote x BALB/cCrSlc)Most homozygous mutant mice were die by 3 weeks of age. |
References | Heparin-binding EGF-like growth factor and ErbB signaling is essential for heart function. Iwamoto R, Yamazaki S, Asakura M, Takashima S, Hasuwa H, Miyado K, Adachi S, Kitakaze M, Hashimoto K, Raab G, Nanba D, Higashiyama S, Hori M, Klagsbrun M, Mekada E Proc. Natl. Acad. Sci. USA, 100, 3221-3226 (2003). 12621152 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Hbegf MGI:96070 | heparin-binding EGF-like growth factor | 18 | Hbegf<tm1.1Mek> MGI:2655703 | targeted mutation 1.1, Eisuke Mekada | |||
lacZ | beta-galactosidase (E. coli) | 18 | |||||
loxP | phage P1 loxP | 18 | loxP | ||||
neo | neomycin resistance gene (E. coli) | 18 | mouse phosphoglycerate kinase promoter (PGK promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 11 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | phage P1 loxP site, E. coli lacZ, poly A, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, poly A, mouse heparin-binding epidermal growth factor-like growth factor genomic DNA |
Research application | Cardiovascular Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. |
Depositor | Eisuke Mekada (Osaka University) |
Strain Status | ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Apr 2006 |
BRC mice in Publications |
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No Data |