Strain Data Sheet

RBRC01308

Strain Information

Image
BRC No.RBRC01308
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hbegf<tm1Mek>/MekRbrc
Former Common nameHB-EGF lox Knock-in mice(BL6) (HBlox#80/B6)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6J
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Eisuke Mekada, Osaka University in 2003.
Strain descriptionB6.Cg-Hbegf<tm1Mek>. Hbegf, heparin-binding EGF like growth factor is a member of the EGF family of growth factor. Hbegf binds to and activates EGF receptor. The targeting vector containing WT HB-EGF cDNA flanked by loxP sites linked to lacZ reporter gene was transfered into TT2 ES cells.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJmsSlc)
References
Heparin-binding EGF-like growth factor and ErbB signaling is essential for heart function.
Iwamoto R, Yamazaki S, Asakura M, Takashima S, Hasuwa H, Miyado K, Adachi S, Kitakaze M, Hashimoto K, Raab G, Nanba D, Higashiyama S, Hori M, Klagsbrun M, Mekada E
Proc. Natl. Acad. Sci. USA, 100, 3221-3226 (2003). 12621152

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Hbegf
MGI:96070		heparin-binding EGF-like growth factor18Hbegf<tm1Mek>
MGI:2655700		targeted mutation 1, Eisuke Mekada
Hbegf heparin-binding epidermal growth factor-like growth factor (mouse)18Hbegf
lacZ beta-galactosidase (E. coli)18
loxP phage P1 loxP18loxP
loxP phage P1 loxP18loxP
neo neomycin resistance gene (E. coli)18 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse heparin-binding epidermal growth factor-like growth factor cDNA, poly A, E. coli LacZ, poly A, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, poly A, mouse heparin-binding epidermal growth factor-like growth factor genomic DNA
    Research applicationCardiovascular Research
    Cell Biology Research
    Cre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
    DepositorEisuke Mekada (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Apr 2006

    BRC mice in Publications

    Sasaki K, Omotuyi OI, Ueda M, Shinohara K, Ueda H.
    NMDA receptor agonists reverse impaired psychomotor and cognitive functions associated with hippocampal Hbegf-deficiency in mice.
    Mol Brain 8(1) 83(2015) 26637193