Strain Information | |
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Image | |
BRC No. | RBRC01308 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Hbegf<tm1Mek>/MekRbrc |
Former Common name | HB-EGF lox Knock-in mice(BL6) (HBlox#80/B6) |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | C57BL/6J |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Dr. Eisuke Mekada, Osaka University in 2003. |
Strain description | B6.Cg-Hbegf<tm1Mek>. Hbegf, heparin-binding EGF like growth factor is a member of the EGF family of growth factor. Hbegf binds to and activates EGF receptor. The targeting vector containing WT HB-EGF cDNA flanked by loxP sites linked to lacZ reporter gene was transfered into TT2 ES cells. |
Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6JJmsSlc) |
References | Proc. Natl. Acad. Sci. USA, 100, 3221-3226 (2003). 12621152 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hbegfheparin-binding epidermal growth factor-like growth factor (mouse)18Hbegf<tm1Mek>targeted mutation 1, Eisuke Mekada Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hbegfheparin-binding epidermal growth factor-like growth factor (mouse)18Hbegf Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)18 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP18loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP18loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)18neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, mouse heparin-binding epidermal growth factor-like growth factor cDNA, poly A, E. coli LacZ, poly A, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, poly A, mouse heparin-binding epidermal growth factor-like growth factor genomic DNA |
Research application | Cardiovascular Research Cell Biology Research Cre/loxP system |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. |
Depositor | Eisuke Mekada (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Mouse of the Month Apr 2006 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Sasaki K, Omotuyi OI, Ueda M, Shinohara K, Ueda H. NMDA receptor agonists reverse impaired psychomotor and cognitive functions associated with hippocampal Hbegf-deficiency in mice. Mol Brain 8(1) 83(2015) 26637193 |