Strain Data Sheet

RBRC01343

Strain Information

Image
BRC No.RBRC01343
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Emx1-cre)2Ito/ItoRbrc
Former Common nameEmx1-Cre Tg2
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Takuji Iwasato and Shigeyoshi Itohara, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6 background.
Strain descriptionEmx1-Cre transgenitc mice, containing a transcriptional unit of a dorsal telencephalon specific promoter (Emx1 promoter) and Cre recombinase gene. Line 1 (RBRC01342), Line 2 (RBRC01343) and Line 3 (RBRC01344). The most suitable cre driver strain is Emx1-cre delta neo knock-in mice (RBRC01345) (no ectopic cre expression is detected).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Dorsal telencephalon-specific expression of Cre recombinase in PAC transgenic mice.
Iwasato T, Nomura R, Ando R, Ikeda T, Tanaka M, Itohara S
Genesis, 38, 130-138 (2004). 15048810

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
cre Phage P1 Cre recombinaseUNcre mouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) promoter, SV40 poly A
nls SV40 large T antigen nuclear localization signal (NLS)UNnls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) promoter, phage P1 Cre recombinase, Simian virus 40 Large T antigen nuclear localization signal (NLS), SV40 poly A
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 38, 130-138 (2004).
    DepositorShigeyoshi Itohara (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month June 2006

    BRC mice in Publications

    Pearson CA, Moore DM, Tucker HO, Dekker JD, Hu H, Miquelajáuregui A, Novitch BG.
    Foxp1 Regulates Neural Stem Cell Self-Renewal and Bias Toward Deep Layer Cortical Fates.
    Cell Rep 30(6) 1964-1981.e3(2020) 32049024