Strain Information | |
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Image | ![]() |
BRC No. | RBRC01347 |
Type | Transgene![]() |
Species | Mus musculus |
Strain name | B6.Cg-Tg(CAG-floxed neo-DT)03Osb |
Former Common name | REP-DT |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Masaru Okabe, Genome Information Research Center, Osaka University in 2003. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. C57BL/6 and DBA/2 mixed background. |
Strain description | CAG-DT conditional transgenic mice (REP-DT mice). The transgene consists of the CAG promoter and the diphtheria toxin A chain (DT) cDNA separated by a floxed neo cassette. When the floxed neo cassette is excised by crossing with Cre mice, DT is expressed. It has been reported that healthy but sterile mice due to a disruption of germ line cells are generated when the REP-DT mice were mated with testis specific cre mice (Prm1-cre). Other tissue/organ-specific expression patterns of the transgene are not clear (Caution: The gene expression pattern in transgenic mice can be different from it of the endogenous gene.) |
Colony maintenance | Carrier x Carrier, Noncarrier [or Crossing to C57BL/6NCrSlc] |
References | Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain. Matsumura H, Hasuwa H, Inoue N, Ikawa M, Okabe M Biochem. Biophys. Res. Commun., 321, 275-279 (2004). 15358172 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
DT | diphtheria toxin A (DT-A) | UN | DT | ||||
loxP | phage P1 loxP | UN | loxP | ||||
loxP | phage P1 loxP | UN | loxP | ||||
neo | neomycin resistance gene (E. coli) | UN | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites, E. coli neo, Corynebacterium diphtheriae diphtheria toxin A fragment DNA, rabbit beta-globin poly A |
Research application | Cre/loxP system General Purpose |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 321, 275-279 (2004).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masaru Okabe (Osaka University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Matsuyama K, Takai S, Shigemura N, Nakatomi M, Kawamoto T, Kataoka S, Toyono T, Seta Y. Ascl1-expressing cell differentiation in initially developed taste buds and taste organoids. Cell Tissue Res (2023) 36781481 |