Strain Data Sheet

RBRC01348

Strain Information

Image
BRC No.RBRC01348
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Trp53<tm1Sia>/Rbrc
Former Common nameICR-p53+/-
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearancealbino [c/c]
Strain developmentDeveloped by Shinichi Aizawa, RIKEN Tsukuba Institute. The TT2 ES cells derived from B6CBAF1 were used to disrupt the p53 gene by homologous recombination. The mutant mice were backcrossed to C57BL/6, ICR and C3H, respectively by Ohtura Niwa, Radiation Biology Center, Kyoto University, to DBA/2 by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University, to MSM by Ryo Kominami, Niigata University Graduate School of Medical and Dental Sciences.
Strain descriptionTrp53 gene knockout mice. A neomycin selection cassette was inserted into the second exon of the Trp53 gene. Homozygous deficient mice can grow after birth, but often suffered from development of tumors in various region of the body. Several congenic strains were generated. C57BL/6 background (RBRC01361), ICR background (RBRC01348), C3H background (RBRC00107), DBA/2 background (RBRC01518), and MSM background (RBRC00815). In C57BL/6 background most of the female homozygotes die in utero (probably, also in MSM background).
Colony maintenanceHeterozygote x Wild-type [or Crossing to Slc:ICR]
References
Enhanced proliferative potential in culture of cells from p53-deficient mice.
Tsukada T, Tomooka Y, Takai S, Ueda Y, Nishikawa S, Yagi T, Tokunaga T, Takeda N, Suda Y, Abe S, et a l
Oncogene, 8, 3313-3322 (1993). 7504233

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Trp53
MGI:98834
transformation related protein 5311Trp53<tm1Sia>
MGI:1926340
targeted mutation 1, Shinichi Aizawa
  • adrenal cortex carcinoma(DisGeNET)

  • adrenocortical carcinoma, hereditary(MedGEN)

  • B-cell chronic lymphocytic leukemia(DisGeNET)
  • more 14 Diseases
  • basal cell carcinoma, susceptibility to, 7(MedGEN)

  • bone marrow failure syndrome 5(MedGEN)

  • bone osteosarcoma(MedGEN)

  • breast carcinoma(DisGeNET)

  • choroid plexus papilloma(MedGEN)

  • colorectal cancer(MedGEN)

  • exocrine pancreatic carcinoma(MedGEN)

  • glioma susceptibility 1(MedGEN)

  • glioma(DisGeNET)

  • hepatocellular carcinoma(MedGEN)

  • hereditary breast carcinoma(MedGEN)

  • Li-Fraumeni syndrome(DisGeNET)

  • nasopharyngeal carcinoma(MedGEN)

  • obsolete Li-Fraumeni syndrome 1(MedGEN)
  • neo neomycin resistance gene (E. coli)11 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal cell proliferation(MP:0000350)

  • decreased cellular sensitivity to ultraviolet irradiation(MP:0008411)

  • increased T cell derived lymphoma incidence(MP:0002024)

  • increased sensitivity to induced cell death(MP:0008943)

  • increased tumor incidence(MP:0002020)
  • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk polyA, mouse p53 genomic DNA
    Research applicationCancer Research
    Cell Biology Research
    Immunology and Inflammation Research
    Mouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Oncogene, 8, 3313-3322 (1993). Please inform Laboratory for Animal Resources and Genetic Engineering, CDB (e mail: mutant.bdr@riken.jp) about the paper when published.
    DepositorOhtsura Niwa (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jun 2005

    BRC mice in Publications

    Okuda A, Kurokawa S, Takehashi M, Maeda A, Fukuda K, Kubo Y, Nogusa H, Takatani-Nakase T, Okuda S, Ueda K, Tanaka S.
    Poly(ADP-ribose) polymerase inhibitors activate the p53 signaling pathway in neural stem/progenitor cells.
    BMC Neurosci 18(1) 14(2017) 28095779
    Maruzuru Y, Koyanagi N, Takemura N, Uematsu S, Matsubara D, Suzuki Y, Arii J, Kato A, Kawaguchi Y.
    p53 Is a Host Cell Regulator during Herpes Simplex Encephalitis.
    J Virol 90(15) 6738-6745(2016) 27170756