Strain Data Sheet

RBRC01351

Strain Information

Image
BRC No.RBRC01351
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129S-Grm2<tm1Nak>/NakRbrc
Former Common nameC57BL/6-Grm2 tm (Nov.2011), GluR2 KO, mGluR2 KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Yokoi et al. (1996). A neomycin resistance cassette replaced 15.3-kb DNA fragment containing exon 2. The Grm2 deficient mice were produced by backcrossing to C57BL/6 strain for 20 times.
Strain descriptionmGluR2 (Grm2, G protein-coupled receptor, family C, group 1, member B) is widely dstributed both presynaptically and postsynaptically in varietry of neural cells. mGluR deficient mice have no behavioral abnormalities. But, a locomotor sensitization to cocaine administration was increased significantly. Moreover, significant impairment in motor coordination was detected. mGluR2 deficient mice were useful to clarify the rule in synaptic regulation of glutamatergic transmission in the neural network.
Colony maintenance
References
Impairment of hippocampal mossy fiber LTD in mice lacking mGluR2.
Yokoi M, Kobayashi K, Manabe T, Takahashi T, Sakaguchi I, Katsuura G, Shigemoto R, Ohishi H, Nomura S, Nakamura K, Nakao K, Katsuki M, Nakanishi S
Science, 273, 645-647 (1996). 8662555

Enhanced cocaine responsiveness and impaired motor coordination in metabotropic glutamate receptor subtype 2 knockout mice.
Morishima Y, Miyakawa T, Furuyashiki T, Tanaka Y, Mizuma H, Nakanishi S
Proc. Natl. Acad. Sci. USA, 102, 4170-4175 (1996). 15753323

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Grm2glutamate receptor, metabotropic 29Grm2targeted mutation 1, Shigetada Nakanishi
neoneomycin resistance gene (E. coli)9herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal GABAergic neuron physiology(MP:0011987)

  • abnormal excitatory postsynaptic potential(MP:0002912)

  • abnormal motor capabilities/coordination/movement(MP:0002066)

  • cocaine preference(MP:0001988)

  • decreased susceptibility to neuronal excitotoxicity(MP:0008236)
  • more 5 phenotypes
  • hyperactivity(MP:0001399)

  • impaired coordination(MP:0001405)

  • increased dopamine level(MP:0001906)

  • nervous system phenotype(MP:0003631)

  • reduced long-term depression(MP:0001475)
  • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse glutamate receptor metabotropic 2 genomic DNA
    Research applicationNeurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 273, 645-647 (1996). Proc Natl Acad Sci USA, 102, 4170-4175 (2005). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorShigetada Nakanishi (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Fribourg M, Moreno JL, Holloway T, Provasi D, Baki L, Mahajan R, Park G, Adney SK, Hatcher C, Eltit JM, Ruta JD, Albizu L, Li Z, Umali A, Shim J, Fabiato A, MacKerell AD Jr, Brezina V, Sealfon SC, Filizola M, González-Maeso J, Logothetis DE.
    Decoding the signaling of a GPCR heteromeric complex reveals a unifying mechanism of action of antipsychotic drugs.
    Cell 147(5) 1011-23(2011) 22118459
    Moreno JL, Muguruza C, Umali A, Mortillo S, Holloway T, Pilar-Cuéllar F, Mocci G, Seto J, Callado LF, Neve RL, Milligan G, Sealfon SC, López-Giménez JF, Meana JJ, Benson DL, González-Maeso J.
    Identification of three residues essential for 5-hydroxytryptamine 2A-metabotropic glutamate 2 (5-HT2A·mGlu2) receptor heteromerization and its psychoactive behavioral function.
    J Biol Chem 287(53) 44301-19(2012) 23129762
    Kim H, Lee Y, Park JY, Kim JE, Kim TK, Choi J, Lee JE, Lee EH, Kim D, Kim KS, Han PL.
    Loss of Adenylyl Cyclase Type-5 in the Dorsal Striatum Produces Autistic-Like Behaviors.
    Mol Neurobiol 54(10) 7994-8008(2017) 27878759
    Moreno JL, Holloway T, Rayannavar V, Sealfon SC, González-Maeso J.
    Chronic treatment with LY341495 decreases 5-HT(2A) receptor binding and hallucinogenic effects of LSD in mice.
    Neurosci Lett 536 69-73(2013) 23333599
    Moreno JL, Holloway T, Albizu L, Sealfon SC, González-Maeso J.
    Metabotropic glutamate mGlu2 receptor is necessary for the pharmacological and behavioral effects induced by hallucinogenic 5-HT2A receptor agonists.
    Neurosci Lett 493(3) 76-9(2011) 21276828
    Moreno JL, Miranda-Azpiazu P, García-Bea A, Younkin J, Cui M, Kozlenkov A, Ben-Ezra A, Voloudakis G, Fakira AK, Baki L, Ge Y, Georgakopoulos A, Morón JA, Milligan G, López-Giménez JF, Robakis NK, Logothetis DE, Meana JJ, González-Maeso J.
    Allosteric signaling through an mGlu2 and 5-HT2A heteromeric receptor complex and its potential contribution to schizophrenia.
    Sci Signal 9(410) ra5(2016) 26758213