Strain Data Sheet

RBRC01356

Strain Information

Image
BRC No.RBRC01356
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Grm6-lacZ)35Kyo
Former Common nameZF35
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shigetada Nakanishi, Kyoto University Faculty of Medicine. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. C57BL/6 semicongenic.
Strain descriptionmGluR6 (glutamate receptor, metabotropic 6) gene is suggested to be responsible for ON responses in both the rod and cone systems. The transgene is mGluR6 gene fused to the beta-galactosidase reporter gene. This strain is useful for investigating the promoter function of the cell-specific and developmentally regulated expression of mGluR6.
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6JJcl]
References
The mGluR6 5' upstream transgene sequence directs a cell-specific and developmentally regulated expression in retinal rod and ON-type cone bipolar cells.
Ueda Y, Iwakabe H, Masu M, Suzuki M, Nakanishi S
J. Neurosci., 17, 3014-3023 (1997). 9096137

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
gpt guanine phosphoribosyltransferaseUNgpt
lacZ beta-galactosidase (E. coli)UN mouse mGluR6 promoter
trpS UNtrpS

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse mGluR6 promoter, E. coli lacZ, SV40 poly A, E. coli gpt, trpS
    Research applicationNeurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 17, 3014-3023 (1997).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorShigetada Nakanishi (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data