Strain Data Sheet

RBRC01358

Strain Information

Image
BRC No.RBRC01358
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Grm2-IL2RA)2Kyo
Former Common nameMG-I2
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Dai Watanabe and Shigetada Nakanishi, Kyoto University Faculty of Medicine in 1998.
Strain descriptionThis transgenic mouse strain expresses the human IL2RA gene under the control of mouse GluR promoter. The IL2RA mRNA was highly expressed in Golgi cells of the cerebellum, granule cells of the main and accessory olfactory bulbs, the pontine nuclei, and neuronal cells of the cerebellum cortex. Homozygous and hemizygous mutant mice are viable and fertile.
Colony maintenanceHomozygous and hemizygous mutant mice are viable and fertile.
References
Ablation of cerebellar Golgi cells disrupts synaptic integration involving GABA inhibition and NMDA receptor activation in motor coordination.
Watanabe D, Inokawa H, Hashimoto K, Suzuki N, Kano M, Shigemoto R, Hirano T, Toyama K, Kaneko S, Yokoi M, Moriyoshi K, Suzuki M, Kobayashi K, Nagatsu T, Kreitman R J, Pastan I, Nakanishi S
Cell, 95, 17-27 (1998). 9778244

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
IL2RA Interleukin-2 receptor α-subunit (human)UNIL2RA mouse mGluR2 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal autopod morphology(MP:0000572)

  • abnormal cerebellar Golgi cell morphology(MP:0009512)

  • abnormal motor coordination/balance(MP:0001516)

  • abnormal nervous system electrophysiology(MP:0002272)

  • athetotic walking movements(MP:0001527)
    • more 1 phenotypes
  • impaired balance(MP:0001525)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse mGluR2 promoter, human IL-2R alpha cDNA, polyA
    Research applicationNeurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell, 95, 17-27 (1998).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorShigetada Nakanishi (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data