Strain Data Sheet

RBRC01362

Strain Information

Image
BRC No.RBRC01362
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(CAG-cat,-Mesp1)1Ysa/YsaRbrc
Former Common nameCAG-CAT-Mesp1
H-2 Haplotype
ES Cell line
Background strainMCH
Appearance
Strain developmentDeveloped by Dr. Yumiko Saga, National Institute of Genetics in 2001. ICR background.
Strain descriptionThe transgene contains CAG promoter and Mesp1 cDNA separated by a floxed chloramphenicol acetyltransferase (CAT) cassette. When the floxed CAT cassette is excised by crossing with Cre mice, Mesp1 is expressed.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mesp1
MGI:107785		mesoderm posterior 1UNMesp1
cat E. coli chloramphenicol acetyltransferase (CAT)UN CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
loxP phage P1 loxPUNloxP
loxP phage P1 loxPUNloxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites, E. coli chloramphenicol acetyltransferase (CAT) gene, Mesp1 cDNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. Within a period of 2 years after deposition of the material, user must contact the DEPOSITOR and notify him a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then user must include DEPOSITOR and/or developer's names as co-authors.
    DepositorYumiko Saga (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data