Strain Data Sheet

RBRC01373

Strain Information

Image
BRC No.RBRC01373
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(CAG-cat,-Dll4)2Ysa/YsaRbrc
Former Common nameCAG-CAT-Dll4#2
H-2 Haplotype
ES Cell line
Background strainICR
Appearancealbino [c/c]
Strain developmentDeveloped by Dr. Hiroki Kokubo, National Institute of Genetics, Japan in 2004. This strain was maintained under ICR background.
Strain descriptionICR.Cg-Tg(CAG-cat,-Dll4)2Ysa. In these transgenic mice, The Dll4, Delta-like 4 gene is expressed after excision of the floxed-CAT gene by Cre-mediated recombination.
Colony maintenanceHomozygote x Hemizygote or Crossing to ICR (Jcl:MCH)
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dll4
MGI:1859388		delta like canonical Notch ligand 4UNDll4
  • Adams-Oliver syndrome 6(MedGEN)
    • cat E. coli chloramphenicol acetyltransferase (CAT)UN CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
    loxP phage P1 loxPUNloxP
    loxP phage P1 loxPUNloxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites, E. coli chloramphenicol acetyltransferase (CAT) gene, mouse Dll4 cDNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Within a period of 2 years after deposition of the material, user must contact the DEPOSITOR and notify him a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then user must include DEPOSITOR and/or developer's names as co-authors.
    DepositorYumiko Saga (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data