Strain Data Sheet

RBRC01386

Strain Information

Image
BRC No.RBRC01386
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;FVB-Tg(ACTA1-cre)AMcle/Rbrc
Former Common nameTransgenic mouse, MuCre-A
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentThe expression construct was created by cloning cDNAs encoding the selected genes and the promoter into a plasmid vector. After verification of the construct by nucleotide sequencing, the transcriptional unit was cleaved out with restriction enzymes, purified with biochemical method and used for microinjection. The offspring were initially examined by PCR genotyping. The in vivo expression was checked by crossing the mice with two Cre-reporter lines.
Strain descriptionMuCreA contains a transcriptional unit consisting of a muscle-specific promoter (alpha-actin promoter) and Cre recombinase gene. The mouse is used to make myogenic-specific knock-outs by crossing with mice that have a floxed gene. The MuCreA produces rapid and early cleavage of floxed genes from all differentiated myogenic cells, including cardiac myocytes and skeletal myotubes.
Colony maintenance
References
Myocardial deletion of Smad4 using a novel alpha skeletal muscle actin Cre recombinase transgenic mouse causes misalignment of the cardiac outflow tract.
Azhar M, Wang P Y, Frugier T, Koishi K, Deng C, Noakes P G, McLennan I S
Int. J. Biol. Sci., 20;6(6):546-555 (2010). 20877696

Health Report

Examination Date / Room / Rack2024/12/09Room:3-2Rack:DSentinel mouse program
2024/09/09Room:3-2Rack:DSentinel mouse program
2024/06/10Room:3-2Rack:DSentinel mouse program
2024/03/11Room:3-2Rack:DSentinel mouse program
2023/12/11Room:3-2Rack:DSentinel mouse program
2023/09/11Room:3-2Rack:DSentinel mouse program
2023/06/13Room:3-2Rack:DSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
cre Phage P1 Cre recombinaseUNcre<Tg(ACTA1-cre)AMcle>
MGI:4833970		transgene insertion A, Ian S. McLennanhuman alpha actin promoter
SV40 polyA signalUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman alpha skeletal muscle actin promoter, phage P1 Cre recombinase, SV40 poly A signal
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Int. J. Biol. Sci., 20;6(6):546-555 (2010).
    DepositorKyoko Koishi (University of Otago)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Nakatsu Y, Matsunaga Y, Nakanishi M, Yamamotoya T, Sano T, Kanematsu T, Asano T.
    Prolyl isomerase Pin1 in skeletal muscles contributes to systemic energy metabolism and exercise capacity through regulating SERCA activity.
    Biochem Biophys Res Commun 715 150001(2024) 38676996
    Fujimoto M, Kitamura H.
    Application of the colorimetric loop-mediated isothermal amplification (LAMP) technique for genotyping Cre-driver mice.
    J Vet Med Sci 84(4) 507-510(2022) 35228407
    Uruno A, Yagishita Y, Katsuoka F, Kitajima Y, Nunomiya A, Nagatomi R, Pi J, Biswal SS, Yamamoto M.
    Nrf2-Mediated Regulation of Skeletal Muscle Glycogen Metabolism.
    Mol Cell Biol 36(11) 1655-72(2016) 27044864
    Uruno A, Furusawa Y, Yagishita Y, Fukutomi T, Muramatsu H, Negishi T, Sugawara A, Kensler TW, Yamamoto M.
    The Keap1-Nrf2 system prevents onset of diabetes mellitus.
    Mol Cell Biol 33(15) 2996-3010(2013) 23716596
    Azhar M, Wang PY, Frugier T, Koishi K, Deng C, Noakes PG, McLennan IS.
    Myocardial deletion of Smad4 using a novel α skeletal muscle actin Cre recombinase transgenic mouse causes misalignment of the cardiac outflow tract.
    Int J Biol Sci 6(6) 546-55(2010) 20877696