Strain Data Sheet

RBRC01387

Strain Information

Image
BRC No.RBRC01387
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ncdn<tm2Afu>/AfuRbrc
Former Common nameNC<lox/lox>
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Akiyoshi Fukamizu, University of Tsukuba in 2004.
Strain descriptionNeurochondrin conditional knock-out mice. The neural tissue-specific neurochondrin is disrupted by Cre-loxP system. Neurochondorin is a cytoplasmic protein involve in neurite outgrowth, chondrocyte differentiation, and bone metabolizm. The nervous system specific deficient mice show that the Ncdh gene is a negative regulator to CaMKII (Ca/calmodulin-dependent protein kinase II) and essential for the spatial learning prosess.
Colony maintenance
References
Neurochondrin negatively regulates CaMKII phosphorylation, and nervous system-specific gene disruption results in epileptic seizure.
Dateki M, Horii T, Kasuya Y, Mochizuki R, Nagao Y, Ishida J, Sugiyama F, Tanimoto K, Yagami K, Imai H, Fukamizu A
J. Biol. Chem., 280, 20503-20508 (2005). 15790563

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ncdn
MGI:1347351		neurochondrin4Ncdn<tm2Afu>
MGI:3586335		targeted mutation 2, Akiyoshi Fukamizu
loxP phage P1 loxP4loxP
loxP phage P1 loxP4loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal astrocyte morphology(MP:0002182)

  • abnormal dentate gyrus morphology(MP:0000812)

  • abnormal hippocampal mossy fiber morphology(MP:0002761)

  • abnormal spatial learning(MP:0001463)

  • impaired coordination(MP:0001405)
    • more 1 phenotypes
  • seizures(MP:0002064)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse Neurochondrin genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 21, 20503-20508 (2005).
    DepositorAkiyoshi Fukamizu (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data