Strain Data Sheet

RBRC01388

Strain Information

Image
BRC No.RBRC01388
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Keap1<tm1Mym>/MymRbrc
Former Common nameKeap1 knockout mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Masayuki Yamamoto, Center for Tsukuba Advanced Research Alliance, University of Tsukuba in 2003. Sequence encoding 8 through 204 of Keap1 was replaced with neomycin and nls-lacZ cassette.
Strain descriptionKeap1 gene is a cytoplasmic protein, acts as a negative regulator of Nrf2 and as a sensor of xenobiotic and oxidative stresses. Double knockout mice of Nrf2 and Keap1 demonstrated that Nrf2 is a primary target of Keap1. Nrf2-Keap1 system is one of the major cellular protection mechanisms against xenobiotic and oxidative stresses. Keap1 homozygous mutant mice exhibit scaly skin, hyperkeratosis in the esophague and forestomatch, and died postnatally.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]. Homozygous mutant mice are postnatal letha.
References
Keap1-null mutation leads to postnatal lethality due to constitutive Nrf2 activation.
Wakabayashi N, Itoh K, Wakabayashi J, Motohashi H, Noda S, Takahashi S, Imakado S, Kotsuji T, Otsuka F, Roop D R, Harada T, Engel J D, Yamamoto M
Nat. Genet., 35, 238-45, (2003). 14517554

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Keap1
MGI:1858732		kelch-like ECH-associated protein 19Keap1<tm1Mym> targeted mutation 1, Masayuki Yamamoto
lacZ beta-galactosidase (E. coli)9
neo neomycin resistance gene (E. coli)9 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
nls SV40 large T antigen nuclear localization signal (NLS)9nls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal esophageal epithelium morphology(MP:0000468)

  • abnormal stomach glandular epithelium morphology(MP:0000473)

  • abnormal stomach non-glandular epithelium morphology(MP:0000472)

  • decreased body size(MP:0001265)

  • decreased body weight(MP:0001262)
    • more 8 phenotypes
  • decreased physiological sensitivity to xenobiotic(MP:0008874)

  • decreased susceptibility to xenobiotic induced morbidity/mortality(MP:0009767)

  • hyperkeratosis(MP:0001242)

  • increased stomach mucosa thickness(MP:0005211)

  • postnatal growth retardation(MP:0001732)

  • postnatal lethality, complete penetrance(MP:0011085)

  • scaly skin(MP:0001192)

  • small stomach(MP:0002691)
    • Detailed phenotype data

    Ordering Information

    Donor DNASimian virus 40 large T antigen nuclear localization signal (NLS), E. coli lacZ, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Keap1 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Genet., 35, 238-45, (2003). The DEPOSITOR should be a co-author in the articles when the users publish their data using these mice for 2 year after deposition to the RIKEN BRC. Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
    DepositorMasayuki Yamamoto (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Oct 2005

    BRC mice in Publications

    Yin Y, Corry KA, Loughran JP, Li J.
    Moderate Nrf2 Activation by Genetic Disruption of Keap1 Has Sex-Specific Effects on Bone Mass in Mice.
    Sci Rep 10(1) 348(2020) 31941926
    Sakai E, Morita M, Ohuchi M, Kido MA, Fukuma Y, Nishishita K, Okamoto K, Itoh K, Yamamoto M, Tsukuba T.
    Effects of deficiency of Kelch-like ECH-associated protein 1 on skeletal organization: a mechanism for diminished nuclear factor of activated T cells cytoplasmic 1 during osteoclastogenesis.
    FASEB J 31(9) 4011-4022(2017) 28515152
    Chen H, Li J, Li H, Hu Y, Tevebaugh W, Yamamoto M, Que J, Chen X.
    Transcript profiling identifies dynamic gene expression patterns and an important role for Nrf2/Keap1 pathway in the developing mouse esophagus.
    PLoS One 7(5) e36504(2012) 22567161