Strain Data Sheet

RBRC01389

Strain Information

Image
BRC No.RBRC01389
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Ahr<tm1(AHR)Mym>/MymRbrc
Former Common nameHuman AhR knock in mouse
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6J
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Takashi Moriguchi et al. in 2001. Human AHR cDNA and neomycin resistant gene were inserted in mouse Ahr gene. This construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. This knock-in mice were backcrossed with C57BL/6J for several times.
Strain descriptionThe aryl hydrocarbon receptor (AhR) (dioxin receptor) is a transcription factor that mediates to catalyze xenobiotic Phase I metabolism. Human AhR knock in mouse, possessing human AHR instead of mouse Ahr, is useful as humanized mouse to predict the biological effects of bioaccumulative environmental toxicants like TCDD in humans.
Colony maintenanceBackcross to C57BL/6 (Heterozyote x C57BL/6JJ)
References
Distinct response to dioxin in an arylhydrocarbon receptor (AHR)-humanized mouse.
Moriguchi T, Motohashi H, Hosoya T, Nakajima O, Takahashi S, Ohsako S, Aoki Y, Nishimura N, Tohyama C, Fujii-Kuriyama Y, Yamamoto M
Proc. Natl. Acad. Sci. USA, 100, 5652-5657 (2003). 12730383

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
AHR aryl-hydrocarbon receptor (human)12AHR
Ahr
MGI:105043		aryl-hydrocarbon receptor12Ahr<tm1(AHR)Mym>
MGI:3776466		targeted mutation 1, Masayuki Yamamoto
  • retinitis pigmentosa 85(MedGEN)
    • neo neomycin resistance gene (E. coli)12 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • decreased physiological sensitivity to xenobiotic(MP:0008874)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman arylhydrocarbon receptor AhR cDNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse arylhydrocarbon receptor AhR genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 100, 5652-5657 (2003). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMasayuki Yamamoto (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data