Strain Data Sheet

RBRC01422

Strain Information

Image
BRC No.RBRC01422
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ehmt1<tm1Yshk>/YshkRbrc
Former Common nameGLP KO, GLP knockout mouse
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9.
Tachibana M, Ueda J, Fukuda M, Takeda N, Ohta T, Iwanari H, Sakihama T, Kodama T, Hamakubo T, Shinkai Y
Genes Dev., 19, 815-826 (2005). 15774718

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ehmt1
MGI:1924933		euchromatic histone methyltransferase 12Ehmt1<tm1Yshk>
MGI:3575505		targeted mutation 1, Yoichi Shinkai
  • Kleefstra syndrome 1(DisGeNET, MedGEN)
    • loxP phage P1 loxP2loxP
    loxP phage P1 loxP2loxP
    neo neomycin resistance gene (E. coli)2 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal associative learning(MP:0002062)

  • abnormal dendrite morphology(MP:0008143)

  • abnormal dendritic spine morphology(MP:0009936)

  • abnormal hippocampus pyramidal cell morphology(MP:0009940)

  • abnormal miniature excitatory postsynaptic currents(MP:0004753)
    • more 20 phenotypes
  • abnormal object recognition memory(MP:0001458)

  • abnormal response to novel object(MP:0003461)

  • abnormal response to novelty(MP:0003107)

  • abnormal sensory capabilities/reflexes/nociception(MP:0002067)

  • abnormal social investigation(MP:0001360)

  • abnormal social/conspecific interaction behavior(MP:0002557)

  • decreased anxiety-related response(MP:0001364)

  • decreased exploration in new environment(MP:0001417)

  • decreased locomotor activity(MP:0001402)

  • decreased vertical activity(MP:0002757)

  • embryonic growth retardation(MP:0003984)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)

  • enhanced paired-pulse facilitation(MP:0002919)

  • impaired contextual conditioning behavior(MP:0009454)

  • impaired cued conditioning behavior(MP:0009456)

  • impaired synaptic plasticity(MP:0001900)

  • incomplete somite formation(MP:0001689)

  • increased anxiety-related response(MP:0001363)

  • increased startle reflex(MP:0001488)

  • open neural tube(MP:0000929)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse PGK (phoshoglycerate kinase) promoter, E. coli neo, mouse GLP genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev., 19, 815-826 (2005). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorYoichi Shinkai (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data