Strain Data Sheet

RBRC01457

Strain Information

Image
BRC No.RBRC01457
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ehmt2<tm1Yshk>/YshkRbrc
Former Common nameG9a-KO, G9a knockout mouse
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis.
Tachibana M, Sugimoto K, Nozaki M, Ueda J, Ohta T, Ohki M, Fukuda M, Takeda N, Niida H, Kato H, Shinkai Y
Genes Dev., 16, 1779-1791 (2002). 12130538

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ehmt2
MGI:2148922		euchromatic histone lysine N-methyltransferase 217Ehmt2<tm1Yshk> targeted mutation 1, Yoichi Shinkai
neo neomycin resistance gene (E. coli)17 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal apoptosis(MP:0001648)

  • abnormal cell physiology(MP:0005621)

  • embryonic growth arrest(MP:0001730)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse PGK (phoshoglycerate kinase) promoter, E. coli neomycin resistance gene, mouse Ehmt2 genomic DNA
    Research applicationCell Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes & Development, 16, 1779-1791 (2002). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorYoichi Shinkai (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data