Strain Data Sheet

RBRC01458

Strain Information

Image
BRC No.RBRC01458
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Dpt<tm1Hsh>/NakaRbrc
Former Common nameDermatopontin KO mice
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Hroshi Shinkai, Chiba University at 2002. A pgk-neo was transfered into ES cells to relpace exon 1 of Dpt gene.
Strain descriptionDermatopontin (Dpt) is a collagen-associated protein, is widely distributed in extracellular matrix. Homozygote mutant mice not exhibit any obvious anatomical abnormality, but the skin elasticity was increased. This strain is useful for study of the mechanism and the process of collagen fibrillogenesis.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)Homozygote mutant mice are fertile.
References
Targeted disruption of dermatopontin causes abnormal collagen fibrillogenesis.
Takeda U, Utani A, Wu J, Adachi E, Koseki H, Taniguchi M, Matsumoto T, Ohashi T, Sato M, Shinkai H
J. Invest. Dermatol., 119, 678-683 (2002). 12230512

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dpt
MGI:1928392		dermatopontin1Dpt<tm1Hsh>
MGI:2387341		targeted mutation 1, Hiroshi Shinkai
neo neomycin resistance gene (E. coli)1 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal cornea posterior stroma morphology(MP:0003094)

  • abnormal cornea stroma morphology(MP:0005300)

  • abnormal cutaneous collagen fibril morphology(MP:0008438)

  • abnormal skin condition(MP:0001191)

  • abnormal skin tensile strength(MP:0005275)
    • more 6 phenotypes
  • decreased cornea stroma thickness(MP:0003092)

  • decreased cornea thickness(MP:0005543)

  • increased total body fat amount(MP:0010024)

  • loose skin(MP:0005421)

  • thin dermal layer(MP:0001244)

  • vision/eye phenotype(MP:0005391)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk polyA, mouse Dpt genomic DNA
    Research applicationCell Biology Research
    Dermatology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Invest. Dermatol., 119, 678-683 (2002).
    DepositorToshinori Nakayama (Chiba University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data