Strain Data Sheet

RBRC01460

Strain Information

Image
BRC No.RBRC01460
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameFVB/N-Tg(P0-cre)2Gth/GvnRbrc
Former Common nameFVB/N-Tg(P0-cre)2Gth/GvnRbrc, P0Cre-B
H-2 Haplotype
ES Cell line
Background strainFVB
Appearancealbino [A/A B/B c/c]
Strain developmentDeveloped by Dr. Marco Giovannini, INSERM, France in 2000. P0Cre transgene fragment consisnting of P1 Cre recomnbinase and 1.1 kb rat P0 promoter was injected into the pronuclei of FVB/N fertilized egges.
Strain descriptionP0Cre contains a transcriptional unit consisting of a Schwann cell specific promoter (myelin protein zero promoter) and Cre recombinase gene. This strain can used to make a inducible knock-out by crossing with other conditional knock out mice that have floxed genes. P0Cre is a driver mouse that can remove floxed genes only in the myelin of the PNS.
Colony maintenanceSibling Mating
References
Conditional biallelic Nf2 mutation in the mouse promotes manifestations of human neurofibromatosis type 2.
Giovannini M, Robanus-Maandag E, van der Valk M, Niwa-Kawakita M, Abramowski V, Goutebroze L, Woodruff J M, Berns A, Thomas G
Genes Dev., 14, 1617-1630 (2000). 10887156

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
cre Phage P1 Cre recombinaseUNcre rat P0 promoter, rabbit beta-globin intron and polyadenylation signal
nls SV40 large T antigen nuclear localization signal (NLS)UNnls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNArat P0 promoter, Simian virus 40 Large T antigen nuclear localization signal (nls), Phage P1 Cre recombinase, rabbit beta-globin intron and polyadenylation signal
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev., 14, 1617-1630 (2000). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT shall keep DEPOSITOR apprised of any results obtained from the BIOLOGICAL RESOURCE in the course of the research, as soon as they are available. Moreover, if the research results in a potential invention or a substance that may have potential commercial value (hereinafter referred as to the "Invention") RECIPIENT will promptly disclose this Invention to DEPOSITOR with samples thereof for DEPOSITOR's internal use.
    RECIPIENT and DEPOSITOR will consult together and agree about the possibility of filing one or more patent application to Invention conceived by one or more employees of Recipients that directly arise out of the research. The Parties will negotiate in good faith the conditions of such patent application according to each party's inventiveness contribution in the Invention.
    In case of such Invention, patentable or not, reduced in practice or developed in the course of the research that could not have been obtained, reduced to practice or developed but from using the Material and/or Confidential Information, RECIPIENT and DEPOSITOR shall confer with one another as regards licensing and commercialization of such Invention to negotiate in good faith the terms of an institutional agreement as regards said matters.
    DepositorMarco Giovannini (INSERM)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data