Strain Data Sheet

RBRC01461

Strain Information

Image
BRC No.RBRC01461
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameFVB/N-Tg(Mbp-cre)9Gvn/GvnRbrc
Former Common nameMBPCre-9
H-2 Haplotype
ES Cell line
Background strainFVB/N
Appearancealbino [a/a B/B C/C]
Strain developmentDeveloped by Drs. Michiko Niwa-Kawakita and Marco Giovannini at INSERM, France in 2000. MbpCre transgene fragment consisnting of Cre recomnbinase and mouse myelin protein promoter was injected into the pronuclei of FVB/N fertilized egges.
Strain descriptionFVB/N-Tg(Mbp-cre)9Gvn. MbpCre contains a transcriptional unit consisting of a myelin specific promoter (myelin basic protein) and Cre recombinase gene. This strain can be used to make a inducible knock out by crossing with other conditional knock out mice that have floxed genes. This transgene is expressed in myelinating oligodenderocytes in the CNS, testis and pituitary gland.
Colony maintenanceSibling Mating
References
Targeted expression of Cre recombinase to myelinating cells of the central nervous system in transgenic mice.
Niwa-Kawakita M, Abramowski V, Kalamarides M, Thomas G, Giovannini M
Genesis, 26, 127-129 (2000). 10686606

Health Report

Examination Date / Room / Rack2024/12/09Room:3-6Rack:BSentinel mouse program
2024/09/09Room:3-6Rack:BSentinel mouse program
2024/06/10Room:3-6Rack:BSentinel mouse program
2024/03/11Room:3-6Rack:BSentinel mouse program
2023/12/11Room:3-6Rack:BSentinel mouse program
2023/09/11Room:3-6Rack:BSentinel mouse program
2023/06/13Room:3-6Rack:BSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
cre Phage P1 Cre recombinaseUNcre mouse MBP promoter, synthetic intron from pOG3231, rabbit beta-globin 3' splicing and polyadenylation signals
nls SV40 large T antigen nuclear localization signal (NLS)UNnls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse MBP genomic DNA, Simian virus 40 Large T antigen nuclear localization signal (nls), phage P1 Cre recombinase gene, synthetic intron from pOG3231, rabbit beta-globin 3' splicing and polyadenylation signals
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 26, 127-129 (2000). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT shall keep DEPOSITOR apprised of any results obtained from the BIOLOGICAL RESOURCE in the course of the research, as soon as they are available. Moreover, if the research results in a potential invention or a substance that may have potential commercial value (hereinafter referred as to the "Invention") RECIPIENT will promptly disclose this Invention to DEPOSITOR with samples thereof for DEPOSITOR's internal use.
    RECIPIENT and DEPOSITOR will consult together and agree about the possibility of filing one or more patent application to Invention conceived by one or more employees of Recipients that directly arise out of the research. The Parties will negotiate in good faith the conditions of such patent application according to each party's inventiveness contribution in the Invention.
    In case of such Invention, patentable or not, reduced in practice or developed in the course of the research that could not have been obtained, reduced to practice or developed but from using the Material and/or Confidential Information, RECIPIENT and DEPOSITOR shall confer with one another as regards licensing and commercialization of such Invention to negotiate in good faith the terms of an institutional agreement as regards said matters.
    DepositorMarco Giovannini (INSERM)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Hiroaki Oizumi, Yuki Miyamoto, Chika Seiwa, Masahiro Yamamoto, Nozomu Yoshioka, Seiichi Iizuka, Tomohiro Torii, Katsuya Ohbuchi, Kazushige Mizoguchi, Junji Yamauchi, Hiroaki Asou
    Lethal Adulthood Myelin Breakdown by Oligodendrocyte-specific Ddx54 Knockout
    iScience 107448(2023)
    Simankova A, Bizen N, Saitoh S, Shibata S, Ohno N, Abe M, Sakimura K, Takebayashi H.
    Ddx20, DEAD box helicase 20, is essential for the differentiation of oligodendrocyte and maintenance of myelin gene expression.
    Glia 69(11) 2559-2574(2021) 34231259
    Miyamoto Y, Torii T, Tanoue A, Yamauchi J.
    VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination.
    Nat Commun 7 13478(2016) 27876794