Strain Data Sheet

RBRC01463

Strain Information

Image
BRC No.RBRC01463
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6(Cg)-Cacna1g<tm1Ksak>/KsakRbrc
Former Common nameCA1G(neo), C57BL/6-Cacna1g<flox/+> -neo/+
H-2 Haplotype
ES Cell lineBruce (gifted by Dr. Colin L. Stewart)
Background strain
Appearance
Strain developmentDeveloped by Dr. Kenji Sakimura, Brain Research Institute, Nigata University in 2002. The targeted allele of this strain was flanked exon 5 with loxP sites and a FRT flanked Neo resistant gene driven by the PGK promotor.
Strain descriptionCacna1g gene is a member of the low-votage-activeted calcium channel that plays a critical role in calcium ion transport in nerve, muscle and edocrine cells. The targeted allele of this strain was flanked exon 5 with loxP sites and a FRT flanked neo resistant gene driven by the PGK promotor.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cacna1g
MGI:1201678		calcium channel, voltage-dependent, T type, alpha 1G subunit11Cacna1g<tm1Ksak> targeted mutation 1, Kenji Skimura
  • spinocerebellar ataxia 42, early-onset, severe, with neurodevelopmental deficits(MedGEN)

  • spinocerebellar ataxia type 42(MedGEN)
    • Frt yeast FRT (flippase recombination target) site11Frt
    Frt yeast FRT (flippase recombination target) site11Frt
    loxP phage P1 loxP11loxP
    loxP phage P1 loxP11loxP
    neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal single cell response(MP:0003463)

  • impaired behavioral response to xenobiotic(MP:0009747)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phospoglycerokianase(pgk) promoter, mouse pgk poly A, E. coli neo, Phage P1 loxP, S. cerevisiae 2μ plasmid FRT (flipase recombination target) sites
    Research applicationCre/loxP system
    Specific Term and Conditions・The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
    ・The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 2 year after provided to the RIKEN BRC.
    ・Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
    DepositorKenji Sakimura (Niigata University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data