Strain Data Sheet

RBRC01491

Strain Information

Image
BRC No.RBRC01491
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Lrrn4<tm1Ami>/Rbrc
Former Common nameNLRR4-KO(B6); B6.Cg-B430119L13Rik<tm>, B6;B6CB-Lrrn4<tm1>/Rbrc
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Takayoshi Bando and Atsushi Miyajima at the University of Tokyo in 2001. The TT2 ES cells originated from an F1 embryo between C57BL/6 and CBA mice were used. The targeting construct contains relpaced exons with the beta-galactosidase and the neomycin resistance genes by homologous reconbination. The NLRR4 deficient mice were backcrossed to C57BL/6JJcl.
Strain descriptionB6.Cg-B430119L13Rik<tm>. NLRR4 is a member of the neuronal leucine-rich repeat protein family. NLRR proteins are type I transmembrane proteins with leucine-rich repeats and a fibronectine type III repeat, and expressed in neuronal tissues. NLRR4 deficient mice have a severe damage to their long-term memory in hippocampus-dependent tasks, while their hippocampal long-term potentiation was intact. NLRR4 plays a role in hippocamus-dependent long-lasting memory.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6J)Homozygous deficient mice are viable and fertile.
References
Neuronal leucine-rich repeat protein 4 functions in hippocampus-dependent long-lasting memory.
Bando T, Sekine K, Kobayashi S, Watabe A M, Rump A, Tanaka M, Suda Y, Kato S, Morikawa Y, Manabe T, Miyajima A
Mol. Cell. Biol., 25, 4166-4175 (2005). 15870286

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Lrrn4
MGI:2445154		leucine rich repeat neuronal 42Lrrn4<tm1Ami>
MGI:5428888		targeted mutation 1, Atsushi Miyajima
lacZ beta-galactosidase (E. coli)2
neo neomycin resistance gene (E. coli)2 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal spatial learning(MP:0001463)

  • behavior/neurological phenotype(MP:0005386)

  • impaired contextual conditioning behavior(MP:0009454)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, E. coli lacZ, poly A, mouse Lrrn4 genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 10, 4160-4175 (2005). The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
    DepositorAtsushi Miyajima (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data