Strain Data Sheet

RBRC01494

Strain Information

Image
BRC No.RBRC01494
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S2-Dppa3<tm1(rtTA)Nkn>/NknRbrc
Former Common namePGC7 KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Toshinobu Nakamura, Toru Kimura and Toru Nakano, Graduate School of Medicine and Frontier Biosciences, Osaka University in 2003. D3 ES cells were used to generate the mutant mice. The mice were crossed to C57BL/6 (C57BL/6 semicongenic).
Strain descriptionDppa3 (PGC7/Stella) gene knockout (rtTA knock-in mice). The start codon of Dppa3 was replaced with rtTA cDNA and floxed PGK-neo cassette. Dppa3 gene plays a critical role in embryonic development as a maternal facotor. Homozygote males mice are fully fertile, but homozygous females are infertile.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc]
References
PGC7/Stella protects against DNA demethylation in early embryogenesis.
Nakamura T, Arai Y, Umehara H, Masuhara M, Kimura T, Taniguchi H, Sekimoto T, Ikawa M, Yoneda Y, Okabe M, Tanaka S, Shiota K, Nakano T
Nat Cell Biol., 9(1):64-71 (2007). 17143267

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dppa3
MGI:1920958		developmental pluripotency-associated 36Dppa3<tm1(rtTA)Nkn> targeted mutation 1, Toru Nakano
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP
neo neomycin resistance gene (E. coli)6 mouse phosphoglycerate kinase promoter (PGK promoter)
rtTA E. coli Reverse tetracycline transactivator (Mutated tetrepressor DNA-binding domain and the transactivation domain of VP-16)6rtTA

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal DNA methylation(MP:0008877)

  • failure of zygotic cell division(MP:0003406)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, rtTA[E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain], mouse developmental pluripotency-associated 3 (PGC7) genomic DNA
    Research applicationCre/loxP system
    Tet system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE.
    DepositorToshinobu Nakamura (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data