Strain Data Sheet

RBRC01509

Strain Information

Image
BRC No.RBRC01509
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Camk2a-cre,-tTA)5Tyag/TyagRbrc
Former Common nameCaM-Cre 5 mice
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Makoto Sanbo and Takeshi Yagi, National Institute for Physiological Sciences, Japan.
Strain descriptionThis transgenic mouse expresses the cre-recombinase under the control of the calcium/calmodulin-dependent protein kinase II alpha promoter. This strain can be used to make an inducible knock-out by crossing with the other genetically-engineeered mice that have floxed genes.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6NCrlCrlj)
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GH Growth hormone polyA (human)UNGH
IRES internal ribosomal entry site (EMCV)UN
cre Phage P1 Cre recombinaseUNcre mouse Camk2a promoter
tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTA

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse CaM Kinase2 promoter, P1 phage hnCre recombinase, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain, human Growth Hormone-poly A
    Research applicationCell Biology Research
    Cre/loxP system
    Tet system
    Specific Term and Conditions1. The RECIPIENT should negotiate with Dr. Takeshi Yagi and Makoto Sanbo in the case of application for any patents with the results from the use of the BIOLOGICAL RESOURCE.
    2. The RECIPIENT should contact with Dr. Takeshi Yagi and Makoto Sanbo in regard to co-authorship.
    DepositorKazuhiro Ikenaka (National Institute for Physiological Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data