Strain Data Sheet

RBRC01515

Strain Information

Image
BRC No.RBRC01515
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(SLC18A3-cre)KMisa/MisaRbrc
Former Common nameVAChT-Cre.Early, (Formerly VAChT-Cre.Fast)
H-2 Haplotype
ES Cell line
Background strainC57BL/6NJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Hidemi Misawa at Tokyo Metropolitan Institute for Neuroscience in 2003. The VaChT-Cre transgene was injected into the pronuclei of fertilized oocytes of C57BL/6 mice.
Strain descriptionTg(SLC18A3-cre)KMisa, RBRC01515, is a transgenic Cre-driver mouse line, in which Cre expression is driven by the 11.3 kb human vesicular acetylcholine transporter (VAChT) promoter (Misawa et al., 2003). The lines showed restricted Cre expression in approximately one half (40-60%) of the postnatal somatic motor neurons and cholinergic neurons of the medial habenular nucleus. Transgenic expression was not observed in visceromotor or other central or peripheral cholinergic neurons. Cre expression began on approximately postnatal day 7 and reached at maximal levels by 5 weeks of age (earlier onset than Tg(SLC18A3-cre)AMisa, RBRC01516). Re-evaluation (Misawa et al., 2016) of the line by using subtype selective markers showed preferred Cre expression in slow-type motor neurons (S- and FR-type) innervating type I or IIa muscle fibers. To avoid confusion the common name of the line was changed from VAChT-Cre.Fast to VAChT-Cre.Early. Ref. Genesis, 54, 568-572 (2016).
Colony maintenancebackcross to C57BL/6 (Hemizygote x C57BL/6NJcl)
ReferencesGenesis, 37, 44-50 (2003). 14502577
Genesis, 54, 568-572 (2016). 27596971

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)UNinternal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcrehuman vesicular acetylcholine transporter

Ordering Information

Donor DNAHuman vesicular acetylcholine transporter (SLC18A)genomic DNA, Phage P1 Cre recombinase, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Jellyfish GFP cDNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. A prior agreement for collaboration with the depositor should be required.
DepositorHidemi Misawa (Kyoritsu College of Pharmacy)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Luo L, Ambrozkiewicz MC, Benseler F, Chen C, Dumontier E, Falkner S, Furlanis E, Gomez AM, Hoshina N, Huang WH, Hutchison MA, Itoh-Maruoka Y, Lavery LA, Li W, Maruo T, Motohashi J, Pai EL, Pelkey KA, Pereira A, Philips T, Sinclair JL, Stogsdill JA, Traunmüller L, Wang J, Wortel J, You W, Abumaria N, Beier KT, Brose N, Burgess HA, Cepko CL, Cloutier JF, Eroglu C, Goebbels S, Kaeser PS, Kay JN, Lu W, Luo L, Mandai K, McBain CJ, Nave KA, Prado MAM, Prado VF, Rothstein J, Rubenstein JLR, Saher G, Sakimura K, Sanes JR, Scheiffele P, Takai Y, Umemori H, Verhage M, Yuzaki M, Zoghbi HY, Kawabe H, Craig AM.
Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors.
Neuron 106(1) 37-65.e5(2020) 32027825