Strain Information | |
---|---|
Image | |
BRC No. | RBRC01516 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6-Tg(SLC18A3-cre)AMisa/MisaRbrc |
Former Common name | VAChT-Cre.Late, (Formerly VAChT-Cre.Slow) |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6NJcl |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Dr. Hidemi Misawa at Tokyo Metropolitan Institute for Neuroscience in 2003. The VaChT-Cre transgene was injected into the pronuclei of fertilized oocytes of C57BL/6 mice. |
Strain description | Tg(SLC18A3-cre)AMisa, RBRC01516, is a transgenic Cre-driver mouse line, in which Cre expression is driven by the 11.3 kb human vesicular acetylcholine transporter (VAChT) promoter (Misawa et al., 2003). The lines showed restricted Cre expression in approximately one half (40-60%) of the postnatal somatic motor neurons and cholinergic neurons of the medial habenular nucleus. Transgenic expression was not observed in visceromotor or other central or peripheral cholinergic neurons. Cre expression began on approximately postnatal day 7 and reached at maximal levels by several months of age (later onset than Tg(SLC18A3-cre)KMisa, RBRC01515). Re-evaluation (Misawa et al., 2016) of the line by using subtype selective markers showed preferred Cre expression in slow-type motor neurons (S- and FR-type) innervating type I or IIa muscle fibers. To avoid confusion the common name of the line was changed from VAChT-Cre.Slow to VAChT-Cre.Late. Ref. Genesis, 54, 568-572 (2016). |
Colony maintenance | backcross to C57BL/6 (Hemizygote x C57BL/6NJcl) |
References | Genesis, 54, 568-572 (2016). 27596971 Genesis, 37, 44-50 (2003). 14502577 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)UN Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)UNinternal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinaseUNcrehuman vesicular acetylcholine transporter |
Ordering Information | |
---|---|
Donor DNA | Human vesicular acetylcholine transporter, Phage P1 Cre recombinase, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Jellyfish GFP cDNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. A prior agreement for collaboration with the depositor should be required. |
Depositor | Hidemi Misawa (Kyoritsu College of Pharmacy) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |